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Product Info Summary
| SKU: | M01746 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-MBD2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01746
BM5471 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-MBD2 Rabbit Monoclonal Antibody catalog # M01746. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MBD2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01746)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ADIH-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human MBD2
Reactive Species
M01746 is reactive to MBD2 in Human, Mouse, Rat
Observed Molecular Weight
49 kDa
Calculated molecular weight
43.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01746 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:50
FC 1:50
Positive Control
WB: human Hacat whole cell, human A431 whole cell, human RT4 whole cell, rat liver tissue, rat RH35 whole cell, mouse liver tissue, mouse liver tissue
Validation Images & Assay Conditions
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Western blot analysis of MBD2 using anti-MBD2 antibody (M01746).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hacat whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human RT4 whole cell lysates,
Lane 4: rat liver tissue lysates,
Lane 5: rat RH35 whole cell lysates,
Lane 6: mouse liver tissue lysates,
Lane 7: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MBD2 antigen affinity purified monoclonal antibody (M01746) at 1:5000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MBD2 at approximately 49 kDa. The expected band size for MBD2 is at 43 kDa.
Specific Publications For Anti-MBD2 Rabbit Monoclonal Antibody (M01746)
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6 Customer Q&As for Anti-MBD2 Rabbit Monoclonal Antibody
Question
What sample was tested in WB and IHC applications with M01746?
Verified customer
Asked: 2020-01-09
Answer
Western blot analysis of MBD2 expression in A375 cell lysate.
Boster Scientific Support
Answered: 2020-01-09
Question
We need to test anti-MBD2 Monoclonal antibody M01746 on mouse testis for research purposes, then I may be interested in using anti-MBD2 Monoclonal antibody M01746 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-30
Answer
The products we sell, including anti-MBD2 Monoclonal antibody M01746, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-30
Question
Would anti-MBD2 Monoclonal antibody M01746 work for WB with testis?
Verified Customer
Verified customer
Asked: 2019-10-15
Answer
According to the expression profile of testis, MBD2 is highly expressed in testis. So, it is likely that anti-MBD2 Monoclonal antibody M01746 will work for WB with testis.
Boster Scientific Support
Answered: 2019-10-15
Question
Is this M01746 anti-MBD2 Monoclonal antibody reactive to the isotypes of MBD2?
J. Johnson
Verified customer
Asked: 2017-12-27
Answer
The immunogen of M01746 anti-MBD2 Monoclonal antibody is A synthesized peptide derived from human MBD2 Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-12-27
Question
Would M01746 anti-MBD2 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
O. Li
Verified customer
Asked: 2017-01-24
Answer
As indicated on the product datasheet, M01746 anti-MBD2 Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-01-24
Question
Is a blocking peptide available for product anti-MBD2 Monoclonal antibody (M01746)?
T. Banerjee
Verified customer
Asked: 2015-06-12
Answer
We do provide the blocking peptide for product anti-MBD2 Monoclonal antibody (M01746). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2015-06-12
