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Product Info Summary
| SKU: | M30934-2 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-MBP Rabbit Monoclonal Antibody
SKU/Catalog Number
M30934-2
BM3871 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-MBP Rabbit Monoclonal Antibody catalog # M30934-2. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MBP Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M30934-2)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
BD-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide
Reactive Species
M30934-2 is reactive to MBP in Human, Mouse, Rat
Observed Molecular Weight
37 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M30934-2 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
Positive Control
WB: Ecoli
Validation Images & Assay Conditions
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Western blot analysis of MBP expression in Ecoli lysate (M30934-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-malE monoclonal antibody (Catalog # M30934-2) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for malE
Specific Publications For Anti-MBP Rabbit Monoclonal Antibody (M30934-2)
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3 Customer Q&As for Anti-MBP Rabbit Monoclonal Antibody
Question
Our lab were well pleased with the WB result of your anti-MBP Rabbit Monoclonal antibody. However we have observed positive staining in hippocampus subthalamic nucleus myelin membrane using this antibody. Is that expected? Could you tell me where is MBP supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-11-14
Answer
From what I have seen in literature, hippocampus subthalamic nucleus does express MBP. Generally MBP expresses in myelin membrane. Regarding which tissues have MBP expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 17974005
Brain, Pubmed ID: 7504278, 7574672, 7685161
Cervix carcinoma, Pubmed ID: 23186163
Embryonic spinal cord, Pubmed ID: 2427738, 2442403
Hippocampus, and Subthalamic nucleus, Pubmed ID: 14702039
Leukemic T-cell, Pubmed ID: 19690332
Boster Scientific Support
Answered: 2017-11-14
Question
We are currently using anti-MBP Rabbit Monoclonal antibody M30934-2 for all species tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says all species. Is it possible that the antibody can work on dog tissues as well?
E. Baker
Verified customer
Asked: 2014-08-11
Answer
The anti-MBP Rabbit Monoclonal antibody (M30934-2) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-08-11
Question
We have seen staining in all species embryonic spinal cord. Any tips? Is anti-MBP Rabbit Monoclonal antibody supposed to stain embryonic spinal cord positively?
A. Collins
Verified customer
Asked: 2013-12-13
Answer
According to literature embryonic spinal cord does express MBP. According to Uniprot.org, MBP is expressed in c1 segment of cervical spinal cord, embryonic spinal cord, brain, amygdala, hippocampus subthalamic nucleus, brain, lung skin, leukemic t-cell, cervix carcinoma, among other tissues. Regarding which tissues have MBP expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 17974005
Brain, Pubmed ID: 7504278, 7574672, 7685161
Cervix carcinoma, Pubmed ID: 23186163
Embryonic spinal cord, Pubmed ID: 2427738, 2442403
Hippocampus, and Subthalamic nucleus, Pubmed ID: 14702039
Leukemic T-cell, Pubmed ID: 19690332
Boster Scientific Support
Answered: 2013-12-13
