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Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
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Anti-MCM8 Antibody Picoband™
Boster Bio Anti-MCM8 Antibody Picoband™ catalog # A06148-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-MCM8 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06148-1)
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
E.coli-derived human MCM8 recombinant protein (Position: N598-M840).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
A06148-1 is reactive to MCM8 in Human, Mouse, Rat
A06148-1 is guaranteed for ELISA, WB Boster Guarantee
Background of MCM8
DNA replication licensing factor MCM8 is a protein that in humans is encoded by the MCM8 gene. The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein contains the central domain that is conserved among the MCM proteins. And this protein has been shown to co-immunoprecipitate with MCM4, 6 and 7, which suggests that it may interact with other MCM proteins and play a role in DNA replication. Alternatively spliced transcript variants encoding distinct isoforms have been described.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Direct ELISA, 0.1-0.5 μg/ml, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MCM8 using anti-MCM8 antibody (A06148-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human placenta tissue lysates,
Lane 4: rat testis tissue lysates,
Lane 5: rat H9C2(2-1) whole cell lysates,
Lane 6: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCM8 antigen affinity purified polyclonal antibody (Catalog # A06148-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCM8 at approximately 94 kDa. The expected band size for MCM8 is at 94 kDa.
Gene/Protein Information For MCM8 (Source: Uniprot.Org, NCBI)
DNA helicase MCM8
C20orf154; chromosome 20 open reading frame 154; dJ967N21.5; DNA replication licensing factor MCM8; MCM8 minichromosome maintenance deficient 8; MGC119522; MGC119523; MGC12866; MGC4816; Minichromosome maintenance 8; minichromosome maintenance complex component 8; REC homolog; REC MCM8 C20orf154, POF10, dJ967N21.5 minichromosome maintenance 8 homologous recombination repair factor DNA helicase MCM8|DNA replication licensing factor MCM8|MCM8 minichromosome maintenance deficient 8|REC homolog|minichromosome maintenance complex component 8*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on MCM8, check out the MCM8 Infographic
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In this infographic you will see the following information for MCM8: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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