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Product Info Summary
| SKU: | M02107 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Mouse |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-MD-2 LY96 Monoclonal Antibody [9F1B1]
SKU/Catalog Number
M02107
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-MD-2 LY96 Monoclonal Antibody [9F1B1] (Catalog # M02107). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
MD-2 monoclonal antibody can be stored at -20°C, stable for one year.
Cite This Product
Anti-MD-2 LY96 Monoclonal Antibody [9F1B1] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02107)
Host
Mouse
Contents
MD-2 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
Clone: 9F1B1
Isotype
IgG1
Immunogen
Mouse monoclonal MD-2 antibody was raised against a recombinant protein corresponding to amino acids 2 to 160 of human MD-2.
Reactive Species
M02107 is reactive to LY96 in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
18.5 kDa
Background of LY96
MD-2 is a member of the Toll/interleukin-1 receptor (TIR) family, a group of proteins that include the Toll-like receptors (TLRs). TLRs are signaling molecules that recognize different pathogen-associated molecular patterns (PAMPs) and serve as an important link between the innate and adaptive immune responses. TLR4, the major signaling receptor for lipopolysaccharide (LPS), requires the binding of MD-2 to its extracellular region for maximal response to LPS. The specificity of this response is determined by the species of MD-2; e.g., human MD-2 transfected into mouse cells can cause mouse TLR4 to react to LPS analogs that are normally antagonistic to human but not mouse TLR4.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02107 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
MD-2 antibody can be used for detection of MD-2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of (A) 25 and (B) 125 ng of MD-2 recombinant protein with MD-2 antibody at 1 μg/mL.
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Immunohistochemistry of MD-2 in human spleen with MD-2 antibody at 2.5 μg/mL.
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Immunofluorescence of MD2 in human spleen tissue with MD2 antibody at 20 μg/mL.
Specific Publications For Anti-MD-2 LY96 Monoclonal Antibody [9F1B1] (M02107)
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