Click image to see more details
Product Info Summary
| SKU: | A10664-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-MyoD family inhibitor MDFI Antibody
SKU/Catalog Number
A10664-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-MyoD family inhibitor MDFI Antibody catalog # A10664-1. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MyoD family inhibitor MDFI Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10664-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human MDFI. AA range:103-152
Reactive Species
A10664-1 is reactive to MDFI in Human, Mouse
Calculated molecular weight
25.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10664-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:10000
Validation Images & Assay Conditions
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Western blot analysis of lysates from Jurkat cells, using MDFI Antibody. The lane on the right is blocked with the synthesized peptide.
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Western Blot analysis of various cells using MDFI Polyclonal Antibody
Specific Publications For Anti-MyoD family inhibitor MDFI Antibody (A10664-1)
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3 Customer Q&As for Anti-MyoD family inhibitor MDFI Antibody
Question
We are currently using anti-MDFI antibody A10664-1 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
The anti-MDFI antibody (A10664-1) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-22
Question
Will A10664-1 anti-MDFI antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-12-21
Answer
You can see on the product datasheet, A10664-1 anti-MDFI antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-12-21
Question
I am interested in to test anti-MDFI antibody A10664-1 on mouse ectocervix for research purposes, then I may be interested in using anti-MDFI antibody A10664-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Moore
Verified customer
Asked: 2016-08-09
Answer
The products we sell, including anti-MDFI antibody A10664-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-08-09
