Anti-MyoD family inhibitor MDFI Antibody

MDFI antibody

Boster Bio Anti-MyoD family inhibitor MDFI Antibody catalog # A10664-1. Tested in WB applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: A10664-1
Size: 100μl
Reactive Species: Human, Mouse
Host: Rabbit
Application: WB

Product Name

Anti-MyoD family inhibitor MDFI Antibody

View all MDFI Antibodies

SKU/Catalog Number

A10664-1

Size

100μl

Form

Liquid

Description

Boster Bio Anti-MyoD family inhibitor MDFI Antibody catalog # A10664-1. Tested in WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MyoD family inhibitor MDFI Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10664-1)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from the Internal region of human MDFI. at AA rangle: 80-160

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A10664-1 is reactive to MDFI in Human, Mouse

Applications

A10664-1 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

25.029kDa

Background of MDFI

Anti-CAND2 antibody is ideal for western blotting, ELISA and IHC. CAND2 is also known as TIP120B, and TATA-binding protein-interacting protein 120B. While both CAND1 (TIP120A) and CAND2 (TIP120B) are TATA-binding proteins and form complexes with various nuclear proteins involved in the control of eukaryotic gene transcription, CAND2 (TIP120B) is expressed specifically in muscle and heart tissue. This is contrary to the ubiquitous expression of CAND1 (TIP120A). TIP120 homologs exist in various higher eukaryotes including D. melanogaster, C. elegans, and A. thaliana. TIP120B is 60% identical in amino acid sequence to TIP120A.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For MDFI (Source: Uniprot.org, NCBI)

Gene Name

MDFI

Full Name

MyoD family inhibitor

Weight

25.029kDa

Superfamily

MDFI family

Alternative Names

I-MF; I-mfa; inhibitor of MyoD family a; MyoD family inhibitor; Myogenic repressor I-mf MDFI I-MF, I-mfa MyoD family inhibitor myoD family inhibitor|inhibitor of MyoD family a|myogenic repressor I-mf

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on MDFI, check out the MDFI Infographic

MDFI infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MDFI: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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3 Customer Q&As for Anti-MyoD family inhibitor MDFI Antibody

Question

We are currently using anti-MDFI antibody A10664-1 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2019-08-22

Answer

The anti-MDFI antibody (A10664-1) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-22

Question

Will A10664-1 anti-MDFI antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2017-12-21

Answer

You can see on the product datasheet, A10664-1 anti-MDFI antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-12-21

Question

I am interested in to test anti-MDFI antibody A10664-1 on mouse ectocervix for research purposes, then I may be interested in using anti-MDFI antibody A10664-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

A. Moore

Verified customer

Asked: 2016-08-09

Answer

The products we sell, including anti-MDFI antibody A10664-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2016-08-09

Order DetailsPrice
A10664-1

100uL

$399

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