Product Info Summary
SKU: | A10664-1 |
---|---|
Size: | 100μl |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-MyoD family inhibitor MDFI Antibody
SKU/Catalog Number
A10664-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-MyoD family inhibitor MDFI Antibody catalog # A10664-1. Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MyoD family inhibitor MDFI Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10664-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from the Internal region of human MDFI. at AA rangle: 80-160
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A10664-1 is reactive to MDFI in Human, Mouse
Applications
A10664-1 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
25.029kDa
Background of MDFI
Anti-CAND2 antibody is ideal for western blotting, ELISA and IHC. CAND2 is also known as TIP120B, and TATA-binding protein-interacting protein 120B. While both CAND1 (TIP120A) and CAND2 (TIP120B) are TATA-binding proteins and form complexes with various nuclear proteins involved in the control of eukaryotic gene transcription, CAND2 (TIP120B) is expressed specifically in muscle and heart tissue. This is contrary to the ubiquitous expression of CAND1 (TIP120A). TIP120 homologs exist in various higher eukaryotes including D. melanogaster, C. elegans, and A. thaliana. TIP120B is 60% identical in amino acid sequence to TIP120A.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis of specific cells using MDFI Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For MDFI (Source: Uniprot.org, NCBI)
Gene Name
MDFI
Full Name
MyoD family inhibitor
Weight
25.029kDa
Superfamily
MDFI family
Alternative Names
I-MF; I-mfa; inhibitor of MyoD family a; MyoD family inhibitor; Myogenic repressor I-mf MDFI I-MF, I-mfa MyoD family inhibitor myoD family inhibitor|inhibitor of MyoD family a|myogenic repressor I-mf
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MDFI, check out the MDFI Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MDFI: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MyoD family inhibitor MDFI Antibody (A10664-1)
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No publications found for A10664-1
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-MyoD family inhibitor MDFI Antibody
Question
We are currently using anti-MDFI antibody A10664-1 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
The anti-MDFI antibody (A10664-1) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-22
Question
Will A10664-1 anti-MDFI antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-12-21
Answer
You can see on the product datasheet, A10664-1 anti-MDFI antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-12-21
Question
I am interested in to test anti-MDFI antibody A10664-1 on mouse ectocervix for research purposes, then I may be interested in using anti-MDFI antibody A10664-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Moore
Verified customer
Asked: 2016-08-09
Answer
The products we sell, including anti-MDFI antibody A10664-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-08-09