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Product Info Summary
| SKU: | A08392-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Wnt8a Antibody Picoband®
SKU/Catalog Number
A08392-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Wnt8a Antibody Picoband® catalog # A08392-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Wnt8a Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08392-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Wnt8a recombinant protein (Position: D206-S339).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A08392-2 is reactive to WNT8A in Human, Mouse, Rat
Observed Molecular Weight
60 kDa
Calculated molecular weight
38.8 kDa
Background of WNT8A
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family, and may be implicated in development of early embryos as well as germ cell tumors. Multiple alternatively spliced transcript variants have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08392-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human U87 whole cell, human SH-SY5Y whole cell, human SW620 whole cell, human Raji whole cell, rat brain tissue, rat heart tissue, rat C6 whole cell, mouse brain tissue, mouse Neuro-2a whole cell
Validation Images & Assay Conditions
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Western blot analysis of Wnt8a using anti-Wnt8a antibody (A08392-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human U87 whole cell lysates,
Lane 2: human SH-SY5Y whole cell lysates,
Lane 3: human SW620 whole cell lysates,
Lane 4: human Raji whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat heart tissue lysates,
Lane 7: rat C6 whole cell lysates,
Lane 8: mouse brain tissue lysates,
Lane 9: mouse Neuro-2a whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Wnt8a antigen affinity purified polyclonal antibody (Catalog # A08392-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Wnt8a at approximately 60KD. The expected band size for Wnt8a is at 60KD.
Specific Publications For Anti-Wnt8a Antibody Picoband® (A08392-2)
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