Product Info Summary
SKU: | A00054-1 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Monkey, Mouse, Rat |
Host: | Rabbit |
Application: | IF, IHC, WB |
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Product info
Product Name
Anti-MDM2 (Ab-166) Antibody
SKU/Catalog Number
A00054-1
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-MDM2 (Ab-166) Antibody (Catalog # A00054-1). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat, Monkey.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MDM2 (Ab-166) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00054-1)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Clone Number
1E12E1
Isotype
IgG
Immunogen
Peptide sequence around aa.164~168 (A-T-S-E-T) derived from Human MDM2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00054-1 is reactive to MDM2 in Human, Monkey, Mouse, Rat
Applications
A00054-1 is guaranteed for IF, IHC, WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
55.233kDa
Background of MDM2/HDM2
This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues
Haupt, Y. et al. (1997) Nature 387, 296-299.
Zhou, B. P. et al. (2001) Nat. Cell Biol. 3, 973-981.
Grossman, S. R. et al. (1998) Mol. Cell 2, 405-415.
Mayo, L.D. and Donner, D.B. (2001) Proc. Natl. Acad. Sci. USA 98, 11598-11603.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 90kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of MDM2 using anti-MDM2 antibody (A00054-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MDM2 antigen affinity purified polyclonal antibody (Catalog # A00054-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for MDM2.
Click image to see more details
Immunofluorescence staining of methanol-fixed Hela cells using MDM2(Ab-166) Antibody #A00054-1.
Click image to see more details
Figure 2. IHC analysis of MDM2 using anti-MDM2 antibody (A00054-1).
MDM2 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MDM2 Antibody (A00054-1) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For MDM2 (Source: Uniprot.org, NCBI)
Gene Name
MDM2
Full Name
E3 ubiquitin-protein ligase Mdm2
Weight
55.233kDa
Superfamily
MDM2/MDM4 family
Alternative Names
ACTFS; HDM2; HDMX; MDM2 oncogene, E3 ubiquitin protein ligase; MDM2 MDM2 ACTFS, HDMX, LSKB, hdm2 MDM2 proto-oncogene E3 ubiquitin-protein ligase Mdm2|MDM2 oncogene, E3 ubiquitin protein ligase|MDM2 proto-oncogene, E3 ubiquitin protein ligase|Mdm2, p53 E3 ubiquitin protein ligase homolog|Mdm2, transformed 3T3 cell double minute 2, p53 binding protein|double minute 2, human homolog of; p53-binding protein|oncoprotein Mdm2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MDM2, check out the MDM2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MDM2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MDM2 (Ab-166) Antibody (A00054-1)
Hello CJ!
A00054-1 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Jiang X,Yuan J,Dou Y,Zeng D, Xiao S.Lipopolysaccharide Affects the Proliferation and Glucose Metabolism of Cervical Cancer Cells Through the FRA1/MDM2/p53 Pathway.Int J Med Sci 2021;18(4): 1030-1038.doi:10. 7150/ijms.47360.
Species: Human
A00054-1 usage in article: APP:WB, SAMPLE:CASKI CELL AND HELA CELL, DILUTION:NA
Epigenetic repression of long non-coding RNA MEG3 mediated by DNMT1 represses the p53 pathway in gliomas
Fra-1 is upregulated in gastric cancer tissues and affects the PI3K/Akt and p53 signaling pathway in gastric cancer
Fra-1 is upregulated in lung cancer tissues and inhibits the apoptosis of lung cancer cells by the P53 signaling pathway
The Oncoprotein HBXIP Modulates the Feedback Loop of MDM2/p53 to Enhance the Growth of Breast Cancer
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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16 Customer Q&As for Anti-MDM2 (Ab-166) Antibody
Question
you antibody to test anti-MDM2 (Ab-166) antibody A00054-1 on human leukemic t-cell for research purposes, then I may be interested in using anti-MDM2 (Ab-166) antibody A00054-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-04-17
Answer
The products we sell, including anti-MDM2 (Ab-166) antibody A00054-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-17
Question
I have a question about product A00054-1, anti-MDM2 (Ab-166) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-17
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00054-1 anti-MDM2 (Ab-166) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-17
Question
We have been able to see staining in human leukemic t-cell. Do you have any suggestions? Is anti-MDM2 (Ab-166) antibody supposed to stain leukemic t-cell positively?
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
From literature leukemic t-cell does express MDM2. From Uniprot.org, MDM2 is expressed in oviduct epithelium, colon, ovarian carcinoma, tongue, rhabdomyosarcoma, leukemic t-cell, erythroleukemia, among other tissues. Regarding which tissues have MDM2 expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 1614537
Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Ovarian carcinoma, Pubmed ID: 8705862
Rhabdomyosarcoma, Pubmed ID: 15489334
Tongue, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-03-30
Question
We bought anti-MDM2 (Ab-166) antibody for IHC on oviduct epithelium in a previous experiment. I am using human, and I plan to use the antibody for WB next. My lab would like examining oviduct epithelium as well as colon in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2020-03-19
Answer
I took a look at the website and datasheets of our anti-MDM2 (Ab-166) antibody and it appears that A00054-1 has been validated on human in both IHC and WB. Thus A00054-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-03-19
Question
Here is the WB image, lot number and protocol we used for leukemic t-cell using anti-MDM2 (Ab-166) antibody A00054-1. Please let me know if you require anything else.
J. Carter
Verified customer
Asked: 2020-02-14
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-14
Question
Will anti-MDM2 (Ab-166) antibody A00054-1 work for WB with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2020-01-23
Answer
According to the expression profile of leukemic t-cell, MDM2 is highly expressed in leukemic t-cell. So, it is likely that anti-MDM2 (Ab-166) antibody A00054-1 will work for WB with leukemic t-cell.
Boster Scientific Support
Answered: 2020-01-23
Question
I see that the anti-MDM2 (Ab-166) antibody A00054-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-26
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-26
Question
Is a blocking peptide available for product anti-MDM2 (Ab-166) antibody (A00054-1)?
Verified Customer
Verified customer
Asked: 2019-12-10
Answer
We do provide the blocking peptide for product anti-MDM2 (Ab-166) antibody (A00054-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-12-10
Question
Does A00054-1 anti-MDM2 (Ab-166) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-11
Answer
You can see on the product datasheet, A00054-1 anti-MDM2 (Ab-166) antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-11
Question
We are currently using anti-MDM2 (Ab-166) antibody A00054-1 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on primate tissues as well?
S. Williams
Verified customer
Asked: 2019-09-30
Answer
The anti-MDM2 (Ab-166) antibody (A00054-1) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-30
Question
My colleagues were satisfied with the WB result of your anti-MDM2 (Ab-166) antibody. However we have been able to see positive staining in erythroleukemia nucleoplasm. cytoplasm. nucleus, using this antibody. Is that expected? Could you tell me where is MDM2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-07-29
Answer
From what I have seen in literature, erythroleukemia does express MDM2. Generally MDM2 expresses in nucleus, nucleoplasm. cytoplasm. nucleus,. Regarding which tissues have MDM2 expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 1614537
Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Ovarian carcinoma, Pubmed ID: 8705862
Rhabdomyosarcoma, Pubmed ID: 15489334
Tongue, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-07-29
Question
Is this A00054-1 anti-MDM2 (Ab-166) antibody reactive to the isotypes of MDM2?
J. Krishna
Verified customer
Asked: 2019-02-15
Answer
The immunogen of A00054-1 anti-MDM2 (Ab-166) antibody is Peptide sequence around aa.164~168 (A-I-S-E-T) derived from Human MDM2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-02-15
Question
I was wanting to use your anti-MDM2 (Ab-166) antibody for WB for human leukemic t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human leukemic t-cell identification?
Verified Customer
Verified customer
Asked: 2018-11-30
Answer
It shows on the product datasheet, A00054-1 anti-MDM2 (Ab-166) antibody has been validated for IF, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-11-30
Question
Is there a BSA free version of anti-MDM2 (Ab-166) antibody A00054-1 available?
Verified Customer
Verified customer
Asked: 2018-07-12
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-MDM2 (Ab-166) antibody A00054-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-07-12
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-MDM2 (Ab-166) antibody A00054-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-06-12
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-06-12
Question
I am interested in using your anti-MDM2 (Ab-166) antibody for cardiac septum morphogenesis studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.
K. Anderson
Verified customer
Asked: 2018-02-08
Answer
I appreciate your inquiry. This A00054-1 anti-MDM2 (Ab-166) antibody is tested on hela cells. It is guaranteed to work for IF, IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-02-08