Anti-MDMX MDM4 Antibody

Product Name

Anti-MDMX MDM4 Antibody

View all MDMX Antibodies

SKU/Catalog Number

A01889-1

Size

100ul

Form

Liquid

Description

Boster Bio Anti-MDMX MDM4 Antibody catalog # A01889-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MDMX MDM4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01889-1)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human MDMX around the non-phosphorylation site of S367.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A01889-1 is reactive to MDM4 in Human, Mouse, Rat

Applications

A01889-1 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

54.864kDa

Background of MDMX

Cleavable component of the cohesin complex, involved in chromosome cohesion during cell cycle, in DNA repair, and in apoptosis. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At metaphase-anaphase transition, this protein is cleaved by separase/ESPL1 and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Also plays a role in apoptosis, via its cleavage by caspase-3/CASP3 or caspase-7/CASP7 during early steps of apoptosis: the C-terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway.

McKay M.J., Genomics 36:305-315(1996).
Sadano H., Biochem. Biophys. Res. Commun. 267:418-422(2000).
Nomura N., DNA Res. 1:223-229(1994).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000

Validation Images & Assay Conditions

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Figure 1. Western blotting validation for Anti-MDMX MDM4 Antibody A01889-1

Western Blot (WB) analysis of 293T cells using MDMX polyclonal antibody.
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Gene/Protein Information For MDM4 (Source: Uniprot.org, NCBI)

Alternative Names

DKFZp781B1423; double minute 4 homolog; Double minute 4 protein; double minute 4, human homolog of; p53-binding protein; HDMX; Mdm2-like p53-binding protein; Mdm4 p53 binding protein homolog (mouse); MDM4 Regulator of P53; MDM4; Mdm4, transformed 3T3 cell double minute 4, p53 binding protein (mouse); Mdm4, transformed 3T3 cell double minute 4, p53 binding protein; MDM4-related protein 1; MDMX; MDMXMGC132766; mouse double minute 4, human homolog of; p53-binding protein; MRP1; p53-binding protein Mdm4; protein Mdm4; Protein Mdmx MDM4 BMFS6, HDMX, MDMX, MRP1 MDM4 regulator of p53 protein Mdm4|MDM4 protein variant G|MDM4 protein variant Y|MDM4, p53 regulator|MDM4-related protein 1|Mdm4 p53 binding protein homolog|double minute 4, human homolog of; p53-binding protein|mdm2-like p53-binding protein|protein Mdmx

For more info on MDM4, check out the MDM4 Infographic

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In this infographic, you will see the following information for MDM4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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