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Product Info Summary
| SKU: | M01380 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human, Mouse |
| Host: | Mouse |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-ME2 Antibody (C-term)
SKU/Catalog Number
M01380
Size
50 µl
Description
Boster Bio Anti-ME2 Antibody (C-term) (Catalog # M01380). Tested in WB, IHC-P application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-ME2 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01380)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
970CT2.1.1.2
Isotype
IgG1,k
Immunogen
This ME2 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 522-556 amino acids from the C-terminal region of human ME2.
Reactive Species
M01380 is reactive to ME2 in Human, Mouse
Calculated molecular weight
65.4 kDa
Background of ME2
Catalyzes the conversion of 25-hydroxyvitamin D3 (25(OH)D) to 1-alpha,25-dihydroxyvitamin D3 (1,25(OH)2D) plays an important role in normal bone growth, calcium metabolism, and tissue differentiation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01380 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:4000
IHC-P: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-ME2 Antibody (C-term) at 1:4000 dilution
Lane 1: HL-60 whole cell lysate
Lane 2: NIH/3T3 whole cell lysate
Lane 3: Jurkat whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 65 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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M01380 staining ME2 in human kidney tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Specific Publications For Anti-ME2 Antibody (C-term) (M01380)
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