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Product Info Summary
| SKU: | A05164 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-ME3 Antibody
SKU/Catalog Number
A05164
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-ME3 Antibody (Catalog # A05164). Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ME3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05164)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human ME3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05164 is reactive to ME3 in Human, Mouse
Applications
A05164 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
111 kDa
Calculated molecular weight
67.068kDa
Background of ME3
Malic enzyme catalyzes the oxidative decarboxylation of malate to pyruvate using either NAD+ or NADP+ as a cofactor. Mammalian tissues contain 3 distinct isoforms of malic enzyme: a cytosolic NADP(+)-dependent isoform, a mitochondrial NADP(+)-dependent isoform, and a mitochondrial NAD(+)-dependent isoform. This gene encodes a mitochondrial NADP(+)-dependent isoform. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.
Loeber G., Biochem. J. 304:687-692(1994).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from 3T3 cells and RAW264.7 cells, using ME3 antibody A05164.
Protein Target Info & Infographic
Gene/Protein Information For ME3 (Source: Uniprot.org, NCBI)
Gene Name
ME3
Full Name
NADP-dependent malic enzyme, mitochondrial
Weight
67.068kDa
Superfamily
malic enzymes family
Alternative Names
EC 1.1.1; EC 1.1.1.40; FLJ34862; malate dehydrogenase; Malic enzyme 3; malic enzyme 3, NADP(+)-dependent, mitochondrial; malic enzyme, NADP+-dependent, mitochondrial; mitochondrial NADP(+)-dependent malic enzyme 3; NADP-dependent malic enzyme, mitochondrial; NADP-ME; pyruvic-malic carboxylase ME3 NADP-ME malic enzyme 3 NADP-dependent malic enzyme, mitochondrial|malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+))|malic enzyme 3, NADP(+)-dependent, mitochondrial|malic enzyme, NADP+-dependent, mitochondrial|mitochondrial NADP(+)-dependent malic enzyme 3|pyruvic-malic carboxylase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ME3, check out the ME3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ME3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ME3 Antibody (A05164)
Hello CJ!
No publications found for A05164
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-ME3 Antibody
Question
Does anti-ME3 antibody A05164 work on feline WB with brain?
Verified Customer
Verified customer
Asked: 2020-04-27
Answer
Our lab technicians have not tested anti-ME3 antibody A05164 on feline. You can run a BLAST between feline and the immunogen sequence of anti-ME3 antibody A05164 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-27
Question
I was wanting to use your anti-ME3 antibody for WB for human brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human brain identification?
Verified Customer
Verified customer
Asked: 2019-11-27
Answer
You can see on the product datasheet, A05164 anti-ME3 antibody has been tested for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-27
Question
We are currently using anti-ME3 antibody A05164 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on horse tissues as well?
K. Miller
Verified customer
Asked: 2015-01-30
Answer
The anti-ME3 antibody (A05164) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-01-30
