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Product Info Summary
| SKU: | A09822 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IP, IF, ICC, WB |
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Product info
Product Name
Anti-MED7 Antibody Picoband®
SKU/Catalog Number
A09822
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-MED7 Antibody Picoband® catalog # A09822. Tested in WB, ICC/IF, IP, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-MED7 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09822)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human MED7 recombinant protein (Position: M1-P233). Human MED7 shares 97% amino acid (aa) sequence identity with mouse MED7.
Reactive Species
A09822 is reactive to MED7 in Human, Mouse, Rat
Observed Molecular Weight
27 kDa
Calculated molecular weight
27.2 kDa
Background of MED7
Mediator of RNA polymerase II transcription subunit 7 is an enzyme that in humans is encoded by the MED7 gene. The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Two transcript variants encoding the same protein have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09822 is guaranteed for ELISA, IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Immunoprecipitation, 0.5-2 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human PC-3 whole cell, human Caco-2 whole cell, human A549 whole cell, human Hela whole cell, rat C6 whole cell, rat PC-12 whole cell, mouse RAW2647 whole cell, mouse NIH/3T3 whole cell
ICC/IF: A549 cell
IP: PC-3 cell
Validation Images & Assay Conditions
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Western blot analysis of MED7 using anti-MED7 antibody (A09822).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human Hela whole cell lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse RAW264.7 whole cell lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MED7 antigen affinity purified polyclonal antibody (Catalog # A09822) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MED7 at approximately 27 kDa. The expected band size for MED7 is at 27 kDa.
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IF analysis of MED7 using anti-MED7 antibody (A09822) and anti-Beta Tubulin antibody (M01857-3).
MED7 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-MED7 Antibody (A09822) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Immunoprecipitating (IP) MED7 in PC-3 whole cell lysate.
Western blot analysis of MED7 using anti-MED7 antibody (A09822);
Lane 1: PC-3 whole cell lysates (30ug);
Lane 2: Rabbit control IgG instead of anti-MED7 antibody in PC-3 whole cell lysate;
Lane 3: anti-MED7 antibody (2μg) + PC-3 whole cell lysate (500μg).
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MED7 antigen affinity purified polyclonal antibody (A09822) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for MED7 at approximately 27 kDa. The expected band size for MED7 is at 27 kDa.
Specific Publications For Anti-MED7 Antibody Picoband® (A09822)
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