|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-MEK6 Antibody|
|Description||Rabbit IgG polyclonal antibody for Dual specificity mitogen-activated protein kinase kinase 6(MAP2K6) detection. Tested with WB in Human;Mouse;Rat.|
|Cite This Product||Anti-MEK6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1921)|
|Replacement Item||This antibody may replace the following items: sc-130204|sc-13069|sc-133230|sc-136982|sc-137106|sc-166727|sc-166728|sc-166746|sc-1991|sc-1992|sc-271779|sc-293108|sc-33124|sc-376627|sc-6073|sc-7994-R|sc-8407|sc-959|sc-960|sc-961 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human MEK6(7-23aa KKRNPGLKIPKEAFEQP), identical to the related mouse sequence and different from the related rat sequence by one amino acid.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Dual specificity mitogen-activated protein kinase kinase 6|
|Molecular Weight||37492 MW|
|Protein Function||Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. With MAP3K3/MKK3, catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinases p38 MAPK11, MAPK12, MAPK13 and MAPK14 and plays an important role in the regulation of cellular responses to cytokines and all kinds of stresses. Especially, MAP2K3/MKK3 and MAP2K6/MKK6 are both essential for the activation of MAPK11 and MAPK13 induced by environmental stress, whereas MAP2K6/MKK6 is the major MAPK11 activator in response to TNF. MAP2K6/MKK6 also phosphorylates and activates PAK6. The p38 MAP kinase signal transduction pathway leads to direct activation of transcription factors. Nuclear targets of p38 MAP kinase include the transcription factors ATF2 and ELK1. Within the p38 MAPK signal transduction pathway, MAP3K6/MKK6 mediates phosphorylation of STAT4 through MAPK14 activation, and is therefore required for STAT4 activation and STAT4-regulated gene expression in response to IL-12 stimulation. The pathway is also crucial for IL-6-induced SOCS3 expression and down-regulation of IL-6-mediated gene induction; and for IFNG- dependent gene transcription. Has a role in osteoclast differentiation through NF-kappa-B transactivation by TNFSF11, and in endochondral ossification and since SOX9 is another likely downstream target of the p38 MAPK pathway. MAP2K6/MKK6 mediates apoptotic cell death in thymocytes. Acts also as a regulator for melanocytes dendricity, through the modulation of Rho family GTPases. .|
|Tissue Specificity||Isoform 2 is only expressed in skeletal muscle. Isoform 1 is expressed in skeletal muscle, heart, and in lesser extent in liver or pancreas. .|
|Sequence Similarities||Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.|
|Subcellular Localization||Nucleus . Cytoplasm . Cytoplasm, cytoskeleton . Binds to microtubules.|
|Alternative Names||Dual specificity mitogen-activated protein kinase kinase 6;MAP kinase kinase 6;MAPKK 6;188.8.131.52;MAPK/ERK kinase 6;MEK 6;Stress-activated protein kinase kinase 3;SAPK kinase 3;SAPKK-3;SAPKK3;MAP2K6;MEK6, MKK6, PRKMK6, SKK3;|
|Research Areas|||signal transduction|protein phosphorylation|ser / thr kinases|mapk pathway| cancer|serine/threonine kinases||
Background for Dual specificity mitogen-activated protein kinase kinase 6
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-MEK6 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-MEK6 Antibody Images
Click the images to enlarge.
Lane 1: Rat Ovary Tissue Lysate
Lane 2: Rat Spleen Tissue Lysate
Lane 3: Rat Brain Tissue Lysate
Lane 4: Rat Heart Tissue Lysate
Lane 5: NIH3I3 Cell Lysate
Lane 6: HELA Cell Lysate
Lane 7: JRUKAT Cell Lysate
Lane 8: MCF-7 Cell Lysate
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,