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Product Info Summary
| SKU: | M02354 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-MEKK2 MAP3K2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M02354
BM4550 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-MEKK2 MAP3K2 Rabbit Monoclonal Antibody catalog # M02354. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MEKK2 MAP3K2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02354)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
FHC-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human MEKK2
Reactive Species
M02354 is reactive to MAP3K2 in Human, Mouse, Rat
Observed Molecular Weight
78 kDa
Calculated molecular weight
69.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02354 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-5000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:30
FC 1:50
Positive Control
WB: human Hela whole cell, human K562 whole cell, human MCF-7 whole cell, human A431 whole cell, rat brain tissue, rat PC-12 whole cell, mouse brain tissue, mouse NIH/3T3 whole cell
IHC: rat brain tissue
Validation Images & Assay Conditions
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Western blot analysis of MAP3K2 using anti-MAP3K2 antibody (M02354).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human A431 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP3K2 antigen affinity purified monoclonal antibody (M02354) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MAP3K2 at approximately 78 kDa. The expected band size for MAP3K2 is at 70 kDa.
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IHC analysis of MAP3K2 using anti-MAP3K2 antibody (M02354).
MAP3K2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-MAP3K2 Antibody (M02354) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-MEKK2 MAP3K2 Rabbit Monoclonal Antibody (M02354)
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4 Customer Q&As for Anti-MEKK2 MAP3K2 Rabbit Monoclonal Antibody
Question
My question regarding product M02354, anti-MEKK2 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-16
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02354 anti-MEKK2 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-16
Question
Would anti-MEKK2 Rabbit Monoclonal antibody M02354 work for IF with t-cell?
D. Moore
Verified customer
Asked: 2015-08-21
Answer
According to the expression profile of t-cell, MAP3K2 is highly expressed in t-cell. So, it is likely that anti-MEKK2 Rabbit Monoclonal antibody M02354 will work for IF with t-cell.
Boster Scientific Support
Answered: 2015-08-21
Question
I was wanting to use your anti-MEKK2 Rabbit Monoclonal antibody for IF for mouse t-cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse t-cell identification?
H. Zhang
Verified customer
Asked: 2014-11-21
Answer
You can see on the product datasheet, M02354 anti-MEKK2 Rabbit Monoclonal antibody has been tested for IP, IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-11-21
Question
Does M02354 anti-MEKK2 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
C. Krishna
Verified customer
Asked: 2014-02-26
Answer
It shows on the product datasheet, M02354 anti-MEKK2 Rabbit Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-02-26
