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Product Info Summary
| SKU: | A07175 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Melanoma-associated antigen 4 MAGEA4 Antibody
SKU/Catalog Number
A07175
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Melanoma-associated antigen 4 MAGEA4 Antibody (Catalog # A07175). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
MAGEA4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-Melanoma-associated antigen 4 MAGEA4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07175)
Host
Rabbit
Contents
MAGEA4 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
MAGEA4 antibody was raised against a 16 amino acid peptide near the amino terminus of human MAGEA4. The immunogen is located within the first 50 amino acids of MAGEA4.
Cross-reactivity
MAGEA4 antibody is human specific. At least four isoforms of MAGEA4 are known to exist.
Reactive Species
A07175 is reactive to MAGEA4 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
34.9 kDa
Background of MAGEA4
MAGEA4 is a member of the melanoma-associated antigen family that consists of a number of antigens recognized by cytotoxic T lymphocytes (1). MAGEA4 may serve as a target for antitumoral vaccination and may play a role in embryonal development and tumor transformation or aspects of tumor progression. It is expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma and have been implicated in some hereditary disorders, such as dyskeratosis congenital (2-4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07175 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
MAGEA4 antibody can be used for detection of MAGEA4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of MAGEA4 in A431 cell lysate with MAGEA4 antibody at 1 μg/ml.
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Immunohistochemistry of MAGEA4 in human breast tumor with MAGEA4 antibody at 5 μg/mL.
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Immunofluorescence of MAGEA4 in human breast cancer tissue with MAGEA4 antibody at 20 μg/ml.
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Immunofluorescence of MAGEA4 in human breast cancer tissue with MAGEA4 antibody at 20 μg/ml.
Green: MAGEA4 Antibody (A07175)
Blue: DAPI staining
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Immunocytochemistry of MAGEA4 in HeLa cells with MAGEA4 antibody at 5 μg/ml.
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Immunofluorescence of MAGEA4 inHeLa cells with MAGEA4 antibody at 20 μg/ml.
Red: MAGEA4 Antibody (A07175)
Blue: DAPI staining
Specific Publications For Anti-Melanoma-associated antigen 4 MAGEA4 Antibody (A07175)
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