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Product Info Summary
| SKU: | A05740 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Melanopsin OPN4 Antibody
SKU/Catalog Number
A05740
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Melanopsin OPN4 Antibody catalog # A05740. Tested in WB, ELISA, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Melanopsin OPN4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05740)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human OPN4. AA range:429-478
Reactive Species
A05740 is reactive to OPN4 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
52.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05740 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-300
ELISA 1:2000-20000
Validation Images & Assay Conditions
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Western Blot analysis of COLO205 cells using Melanopsin Polyclonal Antibody
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Western blot analysis of various lysis using Melanopsin Polyclonal Antibody. Secondary antibody was diluted at 1:20000
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Western blot analysis of lysates from COLO cells, using OPN4 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
Specific Publications For Anti-Melanopsin OPN4 Antibody (A05740)
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Customer Q&As
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4 Customer Q&As for Anti-Melanopsin OPN4 Antibody
Question
I see that the anti-Melanopsin antibody A05740 works with WB, what is the protocol used to produce the result images on the product page?
M. Mangal
Verified customer
Asked: 2019-12-09
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-12-09
Question
We are interested in to test anti-Melanopsin antibody A05740 on human brain brain cortex for research purposes, then I may be interested in using anti-Melanopsin antibody A05740 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-26
Answer
The products we sell, including anti-Melanopsin antibody A05740, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-26
Question
We are currently using anti-Melanopsin antibody A05740 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2017-08-02
Answer
The anti-Melanopsin antibody (A05740) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-08-02
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain brain cortex using anti-Melanopsin antibody A05740. Let me know if you need anything else.
E. Gonzalez
Verified customer
Asked: 2017-05-30
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-05-30
