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Product Info Summary
| SKU: | A07301-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-MESP1 Antibody Picoband®
SKU/Catalog Number
A07301-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MESP1 Antibody Picoband® catalog # A07301-2. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MESP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07301-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human MESP1 recombinant protein (Position: M1-K268).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A07301-2 is reactive to MESP1 in Human
Observed Molecular Weight
29 kDa
Calculated molecular weight
28.5 kDa
Background of MESP1
Mesoderm posterior 1 homolog (mouse) is a protein that in humans is encoded by the MESP1 gene. It is mapped to 15q26.1. MESP1 is a transcription factor that regulates cardiovascular progenitor specification.The MESP1 gene encodes a member of the basic helix-loop-helix (bHLH) family of transcriptional regulatory proteins that may play a role in the development of nascent mesoderm.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07301-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human A549 whole cell, human Hela whole cell, human HEK293 whole cell, human Caco-2 whole cell, human Raji whole cell
Validation Images & Assay Conditions
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Western blot analysis of MESP1 using anti-MESP1 antibody (A07301-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human HEK293 whole cell lysates,
Lane 4: human Caco-2 whole cell lysates,
Lane 5: human Raji whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MESP1 antigen affinity purified polyclonal antibody (Catalog # A07301-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MESP1 at approximately 29KD. The expected band size for MESP1 is at 29KD.
Specific Publications For Anti-MESP1 Antibody Picoband® (A07301-2)
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