Anti-Metabotropic Glutamate Receptor 5/GRM5 Antibody Picoband™

Product Name

Anti-Metabotropic Glutamate Receptor 5/GRM5 Antibody Picoband™

View all GRM5 Antibodies

SKU/Catalog Number

A01338-3

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-Metabotropic Glutamate Receptor 5/GRM5 Antibody Picoband™ catalog # A01338-3. Tested in ELISA, WB applications. This antibody reacts with Human, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-Metabotropic Glutamate Receptor 5/GRM5 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01338-3)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Metabotropic Glutamate Receptor 5/GRM5 recombinant protein (Position: K443-S1050).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01338-3 is reactive to GRM5 in Human, Rat

Applications

A01338-3 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

178 kDa

Calculated molecular weight

132.469kDa

Background of GRM5

Metabotropic glutamate receptor 5?is a?G protein-coupled receptor?that in humans is encoded by the?GRM5 gene. It is mapped to 11q14.2-q14.3. This gene encodes a member of the G-protein coupled receptor 3 protein family. The encoded protein is a metabatropic glutamate receptor, whose signaling activates a phosphatidylinositol-calcium second messenger system. This protein may be involved in the regulation of neural network activity and synaptic plasticity. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. A pseudogene of this gene has been defined on chromosome 11. Alternative splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Rat
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of Metabotropic Glutamate Receptor 5/GRM5 using anti-Metabotropic Glutamate Receptor 5/GRM5 antibody (A01338-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U-87MG whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: rat C6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Metabotropic Glutamate Receptor 5/GRM5 antigen affinity purified polyclonal antibody (Catalog # A01338-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Metabotropic Glutamate Receptor 5/GRM5 at approximately 178 kDa. The expected band size for Metabotropic Glutamate Receptor 5/GRM5 is at 132 kDa.

Gene/Protein Information For GRM5 (Source: Uniprot.org, NCBI)

Alternative Names

glutamate receptor, metabotropic 5; GPRC1EMGLUR5metabotropic glutamate receptor 5; mGlu5; mGluR5 GRM5 GPRC1E, MGLUR5, PPP1R86, mGlu5 glutamate metabotropic receptor 5 metabotropic glutamate receptor 5|glutamate receptor, metabotropic 5|protein phosphatase 1, regulatory subunit 86

For more info on GRM5, check out the GRM5 Infographic

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In this infographic, you will see the following information for GRM5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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