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Product Info Summary
| SKU: | A01338-3 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Metabotropic Glutamate Receptor 5/GRM5 Antibody Picoband®
SKU/Catalog Number
A01338-3
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-Metabotropic Glutamate Receptor 5/GRM5 Antibody Picoband® catalog # A01338-3. Tested in ELISA, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Metabotropic Glutamate Receptor 5/GRM5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01338-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Metabotropic Glutamate Receptor 5/GRM5 recombinant protein (Position: K443-S1050).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01338-3 is reactive to GRM5 in Human, Rat
Observed Molecular Weight
178 kDa
Calculated molecular weight
132.5 kDa
Background of GRM5
Metabotropic glutamate receptor 5?is a?G protein-coupled receptor?that in humans is encoded by the?GRM5 gene. It is mapped to 11q14.2-q14.3. This gene encodes a member of the G-protein coupled receptor 3 protein family. The encoded protein is a metabatropic glutamate receptor, whose signaling activates a phosphatidylinositol-calcium second messenger system. This protein may be involved in the regulation of neural network activity and synaptic plasticity. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. A pseudogene of this gene has been defined on chromosome 11. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01338-3 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human U-87MG whole cell, human 293T whole cell, rat C6 whole cell
Validation Images & Assay Conditions
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Western blot analysis of Metabotropic Glutamate Receptor 5/GRM5 using anti-Metabotropic Glutamate Receptor 5/GRM5 antibody (A01338-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U-87MG whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: rat C6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Metabotropic Glutamate Receptor 5/GRM5 antigen affinity purified polyclonal antibody (Catalog # A01338-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Metabotropic Glutamate Receptor 5/GRM5 at approximately 178 kDa. The expected band size for Metabotropic Glutamate Receptor 5/GRM5 is at 132 kDa.
Specific Publications For Anti-Metabotropic Glutamate Receptor 5/GRM5 Antibody Picoband® (A01338-3)
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