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Product Info Summary
| SKU: | A15360-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-METT5D1/METTL15 Antibody Picoband®
SKU/Catalog Number
A15360-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-METT5D1/METTL15 Antibody Picoband® catalog # A15360-1. Tested in WB,ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-METT5D1/METTL15 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15360-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human METT5D1/METTL15 recombinant protein (Position: R200-L407). Human METTL15 shares 87% amino acid (aa) sequence identity with mouse METTL15.
Reactive Species
A15360-1 is reactive to METTL15 in Human, Mouse, Rat
Observed Molecular Weight
46 kDa
Calculated molecular weight
26.7 kDa
Background of METTL15
Predicted to enable rRNA (cytosine-N4-)-methyltransferase activity. Predicted to be involved in rRNA base methylation. Predicted to be located in mitochondrial matrix. Predicted to be integral component of membrane.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A15360-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human K562 whole cell, human A549 whole cell, human 293T whole cell, human A431 whole cell, rat liver tissue, mouse liver tissue, mouse kidney tissue
Validation Images & Assay Conditions
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Western blot analysis of METT5D1/METTL15 using anti-METT5D1/METTL15 antibody (A15360-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human A431 whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: mouse liver tissue lysates,
Lane 7: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-METT5D1/METTL15 antigen affinity purified polyclonal antibody (Catalog # A15360-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for METT5D1/METTL15 at approximately 46 kDa. The expected band size for METT5D1/METTL15 is at 46 kDa.
Specific Publications For Anti-METT5D1/METTL15 Antibody Picoband® (A15360-1)
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