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Product Info Summary
| SKU: | A14279-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-MettL7A Antibody
SKU/Catalog Number
A14279-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-MettL7A Antibody (Catalog # A14279-1). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-MettL7A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14279-1)
Host
Rabbit
Contents
MettL7A Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
MettL7A antibody was raised against a 13 amino acid synthetic peptide near the center of human MettL7A. The immunogen is located within amino acids 130 - 180 of MettL7A.
Cross-reactivity
At least two isoforms of MettL7A are known to exist. This antibody is predicted to not cross-react with MettL7B.
Reactive Species
A14279-1 is reactive to METTL7A in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
28.3 kDa
Background of METTL7A
MettL7A belongs to the methyltransferase superfamily. It is a probable methyltransferase. Methyltransferase is a type of transferase enzyme which transfers a methyl group from a donor to an acceptor. Often methylation occurs on nucleic bases in DNA or amino acids in protein structures. DNA methylation is often utilized to silence and regulate genes without changing the original DNA sequence. DNA methylation may be necessary for normal growth from embryonic stages in mammals. When mutant embryonic stem cells lacking the murine DNA methyltransferase gene were introduced to a germline of mice they caused a recessive lethal phenotype. Methylation may also be linked to cancer development as methylation of tumor suppressor genes promotes tumorgenesis and metastasis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14279-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
MettL7A antibody can be used for detection of MettL7A by Western blot at 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 2 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of MettL7A in MCF cell lysate with MettL7A antibody at 2 μg/mL.
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Immunocytochemistry of MettL7a in MCF7 cells with MettL7a antibody at 2 μg/mL.
Specific Publications For Anti-MettL7A Antibody (A14279-1)
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