Anti-MFSD3 Antibody Picoband™

Product Name

Anti-MFSD3 Antibody Picoband™

View all Mfsd3 Antibodies

SKU/Catalog Number

A19348-2

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-MFSD3 Antibody Picoband™ catalog # A19348-2. Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-MFSD3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A19348-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Clone Number

1B9

Isotype

IgG

Immunogen

E.coli-derived human MFSD3 recombinant protein (Position: M1-A381). Human MFSD3 shares 79.5% and 78.4% amino acid (aa) sequence identity with mouse and rat MFSD3, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A19348-2 is reactive to Mfsd3 in Human

Applications

A19348-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

43 kDa

Calculated molecular weight

43.165kDa

Background of Mfsd3

MFSD3 belongs to the major facilitator superfamily. The exact functions of MFSD3 remain unknown.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x10⁶ cells, Human
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of MFSD3 using anti-MFSD3 antibody (A19348-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MFSD3 antigen affinity purified polyclonal antibody (Catalog # A19348-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MFSD3 at approximately 43 kDa. The expected band size for MFSD3 is at 43 kDa.

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Figure 2. Flow Cytometry analysis of 293T cells using anti-MFSD3 antibody (A19348-2).
Overlay histogram showing 293T cells stained with A19348-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-MFSD3 Antibody (A19348-2, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For Mfsd3 (Source: Uniprot.org, NCBI)

Alternative Names

major facilitator superfamily domain containing 3; major facilitator superfamily domain-containing protein 3

For more info on Mfsd3, check out the Mfsd3 Infographic

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In this infographic, you will see the following information for Mfsd3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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