Anti-Mimitin NDUFAF2 Antibody

Product Name

Anti-Mimitin NDUFAF2 Antibody

View all NDUFAF2 Antibodies

SKU/Catalog Number

A08718

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Mimitin NDUFAF2 Antibody (Catalog # A08718). Tested in ELISA, WB, IF applications. This antibody reacts with Human.

Storage & Handling

Mimitin antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Mimitin NDUFAF2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08718)

Host

Rabbit

Contents

Mimitin Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Mimitin antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human Mimitin. The immunogen is located within the last 50 amino acids of Mimitin.

Reactive Species

A08718 is reactive to NDUFAF2 in Human

Observed Molecular Weight

68 kDa

Calculated molecular weight

19.9 kDa

Background of NDUFAF2

Mimitin, a small mitochondrial protein, whose transcription is directly stimulated by c-Myc, is highly expressed in 80% of esophageal squamous cell carcinomas (ESCC). Suppression of Mimitin expression by RNA interference had no effect in cancerous cell lines such as human cervical carcinoma or hepatocarcinoma cell lines, but caused a decrease in cell proliferation in human glioblastoma, embryonic lung fibroblastic cells, and ESCC, suggesting Mimitin may play a special role in these types of cells. Mimitin expression is also regulated by MAPK kinases and IL-1, but not through the NF-κB-related pathway. It will interact with the microtubular protein MAP1S and can affect the activities of caspase-3 and -7 in cells stimulated to develop apoptosis. Other experiments suggest that Mimitin also acts as a molecular chaperone for the assembly of the mitochondrial complex I.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A08718 is guaranteed for ELISA, IF, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Mimitin antibody can be used for detection of Mimitin by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunoflourescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information NDUFAF2

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