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Product Info Summary
| SKU: | A07396-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Mint3/APBA3 Antibody Picoband®
SKU/Catalog Number
A07396-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Mint3/APBA3 Antibody Picoband® catalog # A07396-2. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Mint3/APBA3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07396-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Mint3/APBA3 recombinant protein (Position: Q62-E544).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A07396-2 is reactive to APBA3 in Human
Observed Molecular Weight
69 kDa
Calculated molecular weight
61.5 kDa
Background of APBA3
Amyloid beta A4 precursor protein-binding family A member 3 is a protein that in humans is encoded by the APBA3 gene. It is mapped to 19p13.3. The protein encoded by this gene is a member of the X11 protein family. It is an adapter protein that interacts with the Alzheimer's disease amyloid precursor protein. This gene product is believed to be involved in signal transduction processes. This gene is a candidate gene for Alzheimer's disease.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07396-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human HEK293 whole cell, human Hela whole cell, human PC-3 whole cell
Validation Images & Assay Conditions
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Western blot analysis of APBA3 using anti-APBA3 antibody (A07396-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEK293 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human PC-3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APBA3 antigen affinity purified polyclonal antibody (Catalog # A07396-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APBA3 at approximately 69 kDa. The expected band size for APBA3 is at 61 kDa.
Specific Publications For Anti-Mint3/APBA3 Antibody Picoband® (A07396-2)
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1 Customer Q&As for Anti-Mint3/APBA3 Antibody Picoband®
Question
We are currently using anti-Mint3/APBA3 antibody A07396-2 for mouse tissue, and we are content with the ELISA results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-28
Answer
The anti-Mint3/APBA3 antibody (A07396-2) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-28
