Click image to see more details
Product Info Summary
| SKU: | A02172 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Mitofusin-1 MFN1 Antibody
SKU/Catalog Number
A02172
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Mitofusin-1 MFN1 Antibody (Catalog # A02172). Tested in ELISA, WB, ICC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
MFN1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-Mitofusin-1 MFN1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02172)
Host
Rabbit
Contents
MFN1 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
MFN1 antibody was raised against a 17 amino acid peptide near the amino terminus of human MFN1. The immunogen is located within amino acids 20 - 70 of MFN1.
Cross-reactivity
MFN1 antibody is human, mouse and rat reactive. MFN1 antibody is predicted to not cross-react with MFN2.
Reactive Species
A02172 is reactive to MFN1 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
84.2 kDa
Background of MFN1
Mitofusin 1 (MFN1) and the related protein MFN2 are mitochondrial membrane GTPase proteins that play a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes (1,2). MFN1 and MFN2 form homotypic and heterotypic complexes and coordinately regulate mitochondrial fusion and are essential for embryonic development (3). When ectopically expressed, MFN1 inhibits the apoptosis-associated amino-terminal conformation change in the apoptotic protein Bax but not its mitochondrial translocation, indicating that MFN1 is involved in the regulating the activation of Bax on the outer mitochondrial membrane (4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02172 is guaranteed for ELISA, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
MFN1 antibody can be used for detection of MFN1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunocytochemistry at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of MFN1 in A431 cell lysate with MFN1 antibody at 1 μg/ml.
Click image to see more details
Immunocytochemistry of MFN1 in A431 cells with MFN1 antibody at 5 μg/mL.
Specific Publications For Anti-Mitofusin-1 MFN1 Antibody (A02172)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Mitofusin-1 MFN1 Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Mitofusin-1 MFN1 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
