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Product Info Summary
| SKU: | PB9265 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Mitofusin 2/MFN2 Antibody Picoband®
SKU/Catalog Number
PB9265
PB0264 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Mitofusin 2/MFN2 Antibody Picoband® catalog # PB9265. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Mitofusin 2/MFN2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9265)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Mitofusin 2 recombinant protein (Position: V601-R757). Human Mitofusin 2 shares 96% and 95% amino acid (aa) sequence identity with mouse and rat Mitofusin 2, respectively.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9265 is reactive to MFN2 in Human, Mouse, Rat
Observed Molecular Weight
86 kDa
Calculated molecular weight
86.4 kDa
Background of MFN2
Mitofusin-2 is a protein that in humans is encoded by the MFN2 gene. It is mapped to chromosome 1 and encodes a 757-amino acid protein that contains an ATP/GTP-binding site motif. This gene is expressed in many tissues and cell lines such as brain and KG-1 with the highest expression in heart and skeletal muscle. It has been found that MFN2 triggers mitochondrial energization, at least in part, by regulating OXPHOS expression through signals that are independent of its role as a mitochondrial fusion protein. And it contributes to the maintenance and operation of the mitochondrial network. Axonal CMT type 2A and autosomal dominant HMSN VI are caused by MFN2 and mutations in MFN2, which emphasizes its important role of mitochondrial function for both optic atrophies and peripheral neuropathies.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9265 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: HELA Whole Cell, A549 Whole Cell, rat brain tissue, rat heart tissue, rat kidney tissue, mouse brain tissue, mouse heart tissue, mouse kidney tissue, mouse small intestine tissue, mouse NIH3T3 whole cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Mitofusin-2 using anti-Mitofusin-2 antibody (PB9265).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Recombinant Human Mitofusin-2 Protein 0.5ng.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mitofusin-2 antigen affinity purified polyclonal antibody (Catalog # PB9265) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Mitofusin-2 at approximately 45KD. The expected band size for Mitofusin-2 is at 45KD.
Click image to see more details
Western blot analysis of Mitofusin-2 using anti-Mitofusin-2 antibody (PB9265).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: HELA Whole Cell Lysate,
Lane 2: A549 Whole Cell Lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mitofusin-2 antigen affinity purified polyclonal antibody (Catalog # PB9265) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Mitofusin-2 at approximately 86KD. The expected band size for Mitofusin-2 is at 86KD.
Click image to see more details
Western blot analysis of Mitofusin 2 using anti-Mitofusin 2 antibody (PB9265).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat heart tissue lysates,
Lane 3: rat kidney tissue lysates,
Lane 4: mouse brain tissue lysates,
Lane 5: mouse heart tissue lysates,
Lane 6: mouse kidney tissue lysates,
Lane 7: mouse small intestine tissue lysates,
Lane 8: mouse NIH3T3 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mitofusin 2 antigen affinity purified polyclonal antibody (Catalog # PB9265) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Mitofusin 2 at approximately 86KD. The expected band size for Mitofusin 2 is at 86KD.
Specific Publications For Anti-Mitofusin 2/MFN2 Antibody Picoband® (PB9265)
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4 Customer Q&As for Anti-Mitofusin 2/MFN2 Antibody Picoband®
Question
My team were happy with the WB result of your anti-Mitofusin 2/MFN2 antibody. However we have observed positive staining in brain mitochondrion outer membrane using this antibody. Is that expected? Could you tell me where is MFN2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-10-25
Answer
From what I have seen in literature, brain does express MFN2. Generally MFN2 expresses in mitochondrion outer membrane. Regarding which tissues have MFN2 expression, here are a few articles citing expression in various tissues:
Bone marrow, Pubmed ID: 9039502
Brain, Pubmed ID: 14702039
Pancreas, Pubmed ID: 15489334
Testis, Pubmed ID: 17974005
Boster Scientific Support
Answered: 2019-10-25
Question
We are currently using anti-Mitofusin 2/MFN2 antibody PB9265 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
The anti-Mitofusin 2/MFN2 antibody (PB9265) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-16
Question
We have been able to see staining in rat heart left ventricle. Do you have any suggestions? Is anti-Mitofusin 2/MFN2 antibody supposed to stain heart left ventricle positively?
Verified Customer
Verified customer
Asked: 2018-09-14
Answer
Based on literature heart left ventricle does express MFN2. Based on Uniprot.org, MFN2 is expressed in heart left ventricle, bone marrow, brain, pancreas, testis, among other tissues. Regarding which tissues have MFN2 expression, here are a few articles citing expression in various tissues:
Bone marrow, Pubmed ID: 9039502
Brain, Pubmed ID: 14702039
Pancreas, Pubmed ID: 15489334
Testis, Pubmed ID: 17974005
Boster Scientific Support
Answered: 2018-09-14
Question
I am interested in using your anti-Mitofusin 2/MFN2 antibody for protein targeting to mitochondrion studies. Has this antibody been tested with western blotting on rat heart tissue? We would like to see some validation images before ordering.
D. Bhatt
Verified customer
Asked: 2018-04-20
Answer
I appreciate your inquiry. This PB9265 anti-Mitofusin 2/MFN2 antibody is validated on hela whole cell lysate, a549 whole cell lysate, rat heart tissue, brain tissue, kidney tissue, mouse heart tissue, small intestine tissue, nih3t3 whole cell lysates. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-04-20
