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- Table of Contents
MFN1 antibody (M02172-1) performs well in IF, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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IF analysis of MFN1 using anti-MFN1 antibody (M02172-1).
MFN1 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-MFN1 Antibody (M02172-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
MFN1 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-MFN1 Antibody (M02172-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Designing a Western blot for MFN1? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the MFN1 Western Blot GuideBundle Save
Anti-Mitofusin 1 MFN1 Antibody Picoband® (monoclonal, 3H3)
MFN1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Mouse | IF, ICC, WB |
Tested Applications
MFN1 antibody was positively detected by WB in:
human Hela tissue, human HepG2 whole cell,
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
MFN1 antibody was positively detected by ICC/IF in:
U2OS cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human
Immunocytochemistry/Immunofluorescence
2μg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
Human
Tested
Monkey
Predicted
Rabbit
Predicted
Bovine
Predicted
Mouse
Predicted
Rat
Predicted
Sheep
Predicted
Dog
Predicted
Product Information
Product Name
Anti-Mitofusin 1 MFN1 Antibody Picoband® (monoclonal, 3H3)
View all MFN1 Antibodies
View all MFN1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
3H3
Isotype
Mouse IgG2b
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminal of human Mitofusin 1, different from the related mouse and rat sequences by one amino acid.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Observed Molecular Weight
84 kDa
Calculated Molecular Weight
84.2 kDa
Background of MFN1
Mitofusin-1 is a protein that in humans is encoded by the MFN1 gene. It is an 8090 kDa mitochondrial member of the dynamin family of molecules. It is ubiquitously expressed, and found in the outer mitochondrial membrane. The protein encoded by this gene is a mediator of mitochondrial fusion, and thereby contribute to the dynamic balance between fusion and fission that determines mitochondria morphology. MFN1 is known to form oligomers, either with itself or MFN2, and to undergo ubiquitination by MARCH5. MFN1 has two key domains. One is a coiledcoil region that mediates MFN1: MFN1/2 binding, and a second is a GTPase domain whose cleavage of GTP is necessary for membrane fusion. Overexpression of MFN1 caused perinuclear mitochondrial clustering.
Cite This Product
Anti-Mitofusin 1 MFN1 Antibody Picoband® (monoclonal, 3H3) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02172-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Rabbit
96%
High Homology
Cow
96%
High Homology
Mouse
93%
High Homology
Rat
93%
High Homology
Sheep
93%
High Homology
Dog
93%
High Homology
Zebrafish
48%
Low homology
Drosophila
26%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for MFN1 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for MFN1 antibody
Publications
Application:
Species:
Polystyrene microplastics-induced cardiotoxicity in chickens via the ROS-driven NF-κB-NLRP3-GSDMD and AMPK-PGC-1α axes
Published: 2022-06-14
Journal: SCIENCE OF THE TOTAL ENVIRONMENT
Application: WB
Impact Factor: 10.75
Species: Chicken
PMID: 35714743
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Product Promise
We promise Anti-Mitofusin 1 MFN1 Antibody Picoband® (monoclonal, 3H3) performs excellently in relevant applications for IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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M02172-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 17, 2026