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Product Info Summary
| SKU: | A03486 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-MKP-2 DUSP4 Antibody
SKU/Catalog Number
A03486
Size
100μl
Form
Liquid
Description
Boster Bio Anti-MKP-2 DUSP4 Antibody catalog # A03486. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat, Monkey.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MKP-2 DUSP4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03486)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human DUSP4. AA range:81-130
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A03486 is reactive to DUSP4 in Human, Monkey, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
43.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03486 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of HEPG2-UV cells using MKP-2 Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .
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Western blot analysis of lysates from RAW264.7, A549, COS7, and NIH/3T3 cells, using DUSP4 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using DUSP4 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-MKP-2 DUSP4 Antibody (A03486)
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Customer Q&As
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4 Customer Q&As for Anti-MKP-2 DUSP4 Antibody
Question
Would anti-MKP-2 antibody A03486 work for WB with testis?
Verified Customer
Verified customer
Asked: 2019-11-18
Answer
According to the expression profile of testis, DUSP4 is highly expressed in testis. So, it is likely that anti-MKP-2 antibody A03486 will work for WB with testis.
Boster Scientific Support
Answered: 2019-11-18
Question
Can you help my question with product A03486, anti-MKP-2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-21
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03486 anti-MKP-2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-21
Question
Would anti-MKP-2 antibody A03486 work on feline WB with testis?
E. Walker
Verified customer
Asked: 2019-02-04
Answer
Our lab technicians have not validated anti-MKP-2 antibody A03486 on feline. You can run a BLAST between feline and the immunogen sequence of anti-MKP-2 antibody A03486 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline testis in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-02-04
Question
I was wanting to use to test anti-MKP-2 antibody A03486 on monkey testis for research purposes, then I may be interested in using anti-MKP-2 antibody A03486 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-05-23
Answer
The products we sell, including anti-MKP-2 antibody A03486, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-05-23
