Anti-MLF1 Antibody (C-term)

Product Name

Anti-MLF1 Antibody (C-term)

View all Myeloid leukemia factor 1 Antibodies

SKU/Catalog Number

M06772

Size

80 µl

Description

Boster Bio Anti-MLF1 Antibody (C-term) (Catalog # M06772). Tested in WB application(s). This antibody reacts with Human.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-MLF1 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06772)

Host

Rabbit

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

This MLF1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 216-250 amino acids from the C-terminal region of human MLF1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M06772 is reactive to MLF1 in Human

Applications

M06772 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

30.627kDa

Background of Myeloid leukemia factor 1

Involved in lineage commitment of primary hemopoietic progenitors by restricting erythroid formation and enhancing myeloid formation. Interferes with erythropoietin-induced erythroid terminal differentiation by preventing cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. Suppresses RFWD2/COP1 activity via CSN3 which activates p53 and induces cell cycle arrest. Binds DNA and affects the expression of a number of genes so may function as a transcription factor in the nucleus.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:2000

Validation Images & Assay Conditions

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All lanes : Anti-MLF1 Antibody (C-term) at 1:2000 dilution
Lane 1: A431 whole cell lysate
Lane 2: U-2OS whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 31 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

Gene/Protein Information For MLF1 (Source: Uniprot.org, NCBI)

Alternative Names

Myelodysplasia-myeloid leukemia factor 1; myeloid leukemia factor 1 MLF1 myeloid leukemia factor 1 myeloid leukemia factor 1|myelodysplasia-myeloid leukemia factor 1|testis tissue sperm-binding protein Li 49e

For more info on MLF1, check out the MLF1 Infographic

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In this infographic, you will see the following information for MLF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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