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Product Info Summary
| SKU: | M00535 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-MLKL Rabbit Monoclonal Antibody
SKU/Catalog Number
M00535
BM4332 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-MLKL Rabbit Monoclonal Antibody catalog # M00535. Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MLKL Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00535)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
DGB-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human MLKL
Reactive Species
M00535 is reactive to MLKL in Human
Observed Molecular Weight
54 kDa
Calculated molecular weight
54.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00535 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
Positive Control
WB: human Hela whole cell, human PC-3 whole cell, human A549 whole cell
IHC: human colon tissue
Validation Images & Assay Conditions
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Western blot analysis of MLKL using anti-MLKL antibody (M00535).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: human A549 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MLKL antigen affinity purified monoclonal antibody (M00535) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MLKL at approximately 54 kDa. The expected band size for MLKL is at 54 kDa.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human colon, using MLKL Antibody.
Specific Publications For Anti-MLKL Rabbit Monoclonal Antibody (M00535)
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5 Customer Q&As for Anti-MLKL Rabbit Monoclonal Antibody
Question
See attached the WB image, lot number and protocol we used for chondrocyte {eco:0000312 using anti-MLKL Rabbit Monoclonal antibody M00535. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-28
Question
Is a blocking peptide available for product anti-MLKL Rabbit Monoclonal antibody (M00535)?
Verified Customer
Verified customer
Asked: 2020-01-16
Answer
We do provide the blocking peptide for product anti-MLKL Rabbit Monoclonal antibody (M00535). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-01-16
Question
My question regarding product M00535, anti-MLKL Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00535 anti-MLKL Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-28
Question
I am interested in to test anti-MLKL Rabbit Monoclonal antibody M00535 on human chondrocyte {eco:0000312 for research purposes, then I may be interested in using anti-MLKL Rabbit Monoclonal antibody M00535 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
C. Zhang
Verified customer
Asked: 2018-12-10
Answer
The products we sell, including anti-MLKL Rabbit Monoclonal antibody M00535, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-12-10
Question
Is this M00535 anti-MLKL Rabbit Monoclonal antibody reactive to the isotypes of MLKL?
E. Singh
Verified customer
Asked: 2018-01-17
Answer
The immunogen of M00535 anti-MLKL Rabbit Monoclonal antibody is A synthesized peptide derived from human MLKL. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-01-17
