Product Info Summary
SKU: | M00535 |
---|---|
Size: | 100 μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-MLKL Rabbit Monoclonal Antibody
SKU/Catalog Number
M00535
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-MLKL Rabbit Monoclonal Antibody catalog # M00535. Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MLKL Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00535)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
DGB-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human MLKL
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
Reactive Species
M00535 is reactive to Mlkl in Human
Applications
M00535 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
40 kDa
Calculated molecular weight
54.317kDa
Background of Mlkl
Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 50-200
Validation Images & Assay Conditions
Click image to see more details
All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
Click image to see more details
Western blot analysis of MLKL expression in HUVEC cell lysate.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human colon, using MLKL Antibody.
Protein Target Info & Infographic
Gene/Protein Information For Mlkl (Source: Uniprot.org, NCBI)
Gene Name
Mlkl
Full Name
Mixed lineage kinase domain-like protein
Weight
54.317kDa
Superfamily
protein kinase superfamily
Alternative Names
FLJ34389; mixed lineage kinase domain-like protein; mixed lineage kinase domain-like; MLKL Mlkl|9130019I15Rik|mixed lineage kinase domain-like|mixed lineage kinase domain-like protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Mlkl, check out the Mlkl Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Mlkl: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MLKL Rabbit Monoclonal Antibody (M00535)
Hello CJ!
No publications found for M00535
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-MLKL Rabbit Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Submit A Review
Be the first to review Anti-MLKL Rabbit Monoclonal Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
5 Customer Q&As for Anti-MLKL Rabbit Monoclonal Antibody
Question
See attached the WB image, lot number and protocol we used for chondrocyte {eco:0000312 using anti-MLKL Rabbit Monoclonal antibody M00535. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-28
Question
Is a blocking peptide available for product anti-MLKL Rabbit Monoclonal antibody (M00535)?
Verified Customer
Verified customer
Asked: 2020-01-16
Answer
We do provide the blocking peptide for product anti-MLKL Rabbit Monoclonal antibody (M00535). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-16
Question
My question regarding product M00535, anti-MLKL Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00535 anti-MLKL Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-28
Question
I am interested in to test anti-MLKL Rabbit Monoclonal antibody M00535 on human chondrocyte {eco:0000312 for research purposes, then I may be interested in using anti-MLKL Rabbit Monoclonal antibody M00535 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
C. Zhang
Verified customer
Asked: 2018-12-10
Answer
The products we sell, including anti-MLKL Rabbit Monoclonal antibody M00535, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-12-10
Question
Is this M00535 anti-MLKL Rabbit Monoclonal antibody reactive to the isotypes of MLKL?
E. Singh
Verified customer
Asked: 2018-01-17
Answer
The immunogen of M00535 anti-MLKL Rabbit Monoclonal antibody is A synthesized peptide derived from human MLKL. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-01-17