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Product Info Summary
| SKU: | A04065 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IF, WB |
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Product info
Product Name
Anti-MME Antibody (Center)
View all Neprilysin/CD10 Antibodies
SKU/Catalog Number
A04065
Size
80 µl
Description
Boster Bio Anti-MME Antibody (Center) (Catalog # A04065). Tested in WB, IF application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-MME Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04065)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This MME antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 274-302 amino acids from the Central region of human MME.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04065 is reactive to MME in Human, Mouse
Reconstitution
Calculated molecular weight
85514 Da
Background of Neprilysin/CD10
MME is a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). This protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. It is a oprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04065 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:10-1:50
WB: 1:1000
Validation Images & Assay Conditions
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Confocal immunofluorescent analysis of MME Antibody (Center) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
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Western blot analysis of lysates from Ramos cell line,mouse kidney and rat kidney tissue (from left to right),using Neprilysin Antibody (Center) (Cat. #A04065). A04065 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Protein Target Info & Infographic
Gene/Protein Information For MME (Source: Uniprot.org, NCBI)
Gene Name
MME
Full Name
Neprilysin
Weight
85514 Da
Superfamily
peptidase M13 family
Alternative Names
Neprilysin, Atriopeptidase, Common acute lymphocytic leukemia antigen, CALLA, Enkephalinase, Neutral endopeptidase 2411, NEP, Neutral endopeptidase, Skin fibroblast elastase, SFE, CD10, MME, EPN MME CALLA, CD10, CMT2T, NEP, SCA43, SFE membrane metalloendopeptidase neprilysin|atriopeptidase|common acute lymphocytic leukemia |membrane metallo-endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10)|membrane metallo-endopeptidase variant 1|membrane metallo-endopeptidase variant 2|neprilysin-390|neprilysin-411|neutral endopeptidase 24.11|skin fibroblast elastase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MME, check out the MME Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MME: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MME Antibody (Center) (A04065)
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