Click image to see more details
Product Info Summary
| SKU: | A01520-2 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Mmp12 Antibody Picoband®
SKU/Catalog Number
A01520-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-Mmp12 Antibody Picoband® catalog # A11525-3. Tested in WB, ELISA applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Mmp12 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01520-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
IgG
Immunogen
E.coli-derived mouse Mmp12 recombinant protein (Position: K237-H442). Mouse Mmp12 shares 62.7% and 90.3% amino acid (aa) sequence identity with mouse and rat Mmp12, respectively.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01520-2 is reactive to Mmp12 in Mouse
Observed Molecular Weight
45 kDa
Calculated molecular weight
55.0 kDa
Background of Mmp12
Matrix metalloproteinase-12 (MMP-12) also known as macrophage metalloelastase (MME) or macrophage elastase (ME) is an enzyme that in humans is encoded by the MMP12 gene. This gene encodes a member of the matrix metalloproteinase family of extracellular matrix-degrading enzymes that are involved in tissue remodeling, wound repair, progression of atherosclerosis and tumor invasion. The encoded preproprotein undergoes proteolytic processing to generate a mature, zinc-dependent endopeptidase enzyme. Mice lacking the encoded protein have a diminished capacity to degrade extracellular matrix components, do not develop emphysema in response to long-term exposure to cigarette smoke, and exhibit impaired clearance and increased mortality upon bacterial infection. This gene is located in a cluster of other matrix metalloproteinase genes on chromosome 9. Alternate splicing generates multiple transcript variants encoding distinct isoforms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01520-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: mouse thymus tissue, mouse spleen tissue
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Mmp12 using anti-Mmp12 antibody (A01520-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse thymus tissue lysates,
Lane 2: mouse spleen tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mmp12 antigen affinity purified polyclonal antibody (Catalog # A01520-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Mmp12 at approximately 45 kDa. The expected band size for Mmp12 is at 55 kDa.
Specific Publications For Anti-Mmp12 Antibody Picoband® (A01520-2)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Mmp12 Antibody Picoband®?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Mmp12 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
