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MMP2 antibody (M00286-1) performs well in IHC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 48 Publications

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m00286 1 mmp2 primary antibodies ihc testing 1

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IHC analysis of MMP2 using anti-MMP2 antibody (M00286-1).
MMP2 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-MMP2 Antibody (M00286-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Anti-MMP2 Rabbit Monoclonal Antibody

MMP2 antibody

48 Publications

Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	IHC, WB

Tested Applications

MMP2 antibody was positively detected by WB in:

human HepG2 whole cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

MMP2 antibody was positively detected by IHC in:

human breast cancer tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Human

Tested

Recommend

Product Information

Product Name

Anti-MMP2 Rabbit Monoclonal Antibody
View all MMP2 Antibodies

Form

Liquid

Uniprot

P08253

Gene ID

4313

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Monoclonal

Clone Number

BBA-13

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human MMP2

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Observed Molecular Weight

95 kDa

Calculated Molecular Weight

73.9 kDa

Cite This Product

Anti-MMP2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00286-1)

Protocols

Western Blot protocol for MMP2 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for MMP2 antibody

Review Protocol

Publications

Resveratrol suppresses GATA4/Sortilin pathway to attenuate vascular smooth muscle cell senescence-associated secretory phenotype and vascular aging-related atherosclerosis

Published: 2026-06-16

Journal: CELLULAR SIGNALLING

Application: WB

Impact Factor: 4.70

Species: Rat

PMID: 42303194

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Free Secondary Antibody

Anti-MMP2 Rabbit Monoclonal Antibody

MMP2 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Resveratrol suppresses GATA4/Sortilin pathway to attenuate vascular smooth muscle cell senescence-associated secretory phenotype and vascular aging-related atherosclerosis

Platycodin D alleviates idiopathic pulmonary fibrosis by targeting PPP2R1A and regulating the PP2A-mediated PI3K/Akt pathway to inhibit fibroblast transdifferentiation

MiR-18a-5p Attenuates Oxidative Stress and Inhibits Lipid Accumulation in Alcoholic Fatty Liver by Activating the CYP1A1-PPAR Axis

DNMT3A-dependent suppression of HIF-1α alleviates myopia by reducing scleral ferroptosis and fibroblast extracellular matrix remodeling

Development of an Effective Drug Carrier for Targeted Therapy in Lung Adenocarcinoma Using ROS-responsive Micelles

An inhalable composite particulate system for targeted delivery of therapeutics deep into small airways: in vitro and in vivo evaluation

Apigenin prevents hypertensive vascular remodeling by regulating the TP53 pathway

Downregulation of GFPT2 enhances cisplatin chemotherapy sensitivity in STK11/KRAS mutant non-small cell lung cancer by regulating the hexosamine biosynthesis pathway, resisting tumor growth

Alkannin Induces G2/M-Phase Arrest, Apoptosis, and Inhibition of Invasion by Targeting GSK3β in Esophageal Squamous Cell Carcinoma

The expression and role of SUZ12 in lung adenocarcinoma

Oral Piwi-Interacting RNA Delivery Mediated by Green Tea-Derived Exosome-Like Nanovesicles for the Treatment of Aortic Dissection

Methotrexate-modified docetaxel liposome targeting with ginsenoside Rh2 as a membrane stabilizer for the treatment of ovarian cancer

Trophoblast cellular adhesion molecule expression regulated by different genes and their correlation with pregnancy-induced hypertension

Enterolactone and trabectedin suppress epithelial ovarian cancer synergistically via upregulating THBS1

Licoricidin combats gastric cancer by targeting the ICMT/Ras pathway in vitro and in vivo.

MiR-31 improves spinal cord injury in mice by promoting the migration of bone marrow mesenchymal stem cells

Chronic exposure to microcystin-leucine-arginine induces epithelial hyperplasia and inflammation in the mouse bladder

Ibrolipim attenuates early-stage nephropathy in diet-induced diabetic minipigs: Focus on oxidative stress and fibrogenesis

Decoy receptor 2 mediation of the senescent phenotype of tubular cells by interacting with peroxiredoxin 1 presents a novel mechanism of renal fibrosis in diabetic nephropathy

Low expression of RECK in oral squamous cell carcinoma patients induces a shorter survival rate through an imbalance of RECK/MMPs

The overexpression of uPA promotes the proliferation and fibrinolytic activity of human umbilical vein endothelial cells

Dioscin-6’-O-acetate impairs migration of lung cancer cells through attenuations of MMP-2 and MMP-9 via NF-κB suppression

Blockage of High-Affinity Choline Transporter Increases Visceral Hypersensitivity in Rats with Chronic Stress

Silencing of lncRNA ZFAS1 inhibits malignancies by blocking Wnt/β-catenin signaling in gastric cancer cells

Enhancing E-cadherin expression via promoter-targeted miR-373 suppresses bladder cancer cells growth and metastasis

AG1296 enhances plaque stability via inhibiting inflammatory responses and decreasing MMP-2 and MMP-9 expression in ApoE−/− mice

Inhibitory effects of doxycycline on the onset and progression of abdominal aortic aneurysm and its related mechanisms

In vivo inhibitory activity of andrographolide derivative ADN-9 against liver cancer and its mechanisms involved in inhibition of tumor angiogenesis

Norcantharidin enhances TIMP‑2 anti‑vasculogenic mimicry activity for human gallbladder cancers through downregulating MMP‑2 and MT1‑MMP

Intermittent cold stress enhances features of atherosclerotic plaque instability in apolipoprotein E‑deficient mice

Kallistatin, a novel anti-angiogenesis agent, inhibits angiogenesis via inhibition of the NF-κB signaling pathway

Norcantharidin inhibits tumor growth and vasculogenic mimicry of human gallbladder carcinomas by suppression of the PI3-K/MMPs/Ln-5γ2 signaling pathway

Molecular mechanism of inhibitory effects of CD59 gene on atherosclerosis in ApoE (−/−) mice

Establishment of a new representative model of human ovarian cancer in mice

Clinical Study of Tumor Angiogenesis and Perfusion Imaging Using Multi-slice Spiral Computed Tomography for Breast Cancer

Nodal promotes aggressive phenotype via Snail-mediated epithelial–mesenchymal transition in murine melanoma

Acipimox attenuates atherosclerosis and enhances plaque stability in ApoE-deficient mice fed a palmitate-rich diet

Efficacy of intravitreal captopril on oxygen-induced retinopathy in mice

Inhibition of tumor growth and vasculogenic mimicry by curcumin through down-regulation of the EphA2/PI3K/MMP pathway in a murine choroidal melanoma model

Downregulation of Id1 by small interfering RNA in prostate cancer PC3 cells in vivo and in vitro

Knockdown of ribonuclease inhibitor expression with siRNA in non-invasive bladder cancer cell line BIU-87 promotes growth and metastasis potentials

Expression of MMP, HPSE, and FAP in Stroma Promoted Corneal Neovascularization Induced by Different Etiological Factors

19-peptide, a fragment of tumstatin, inhibits the growth of poorly differentiated gastric carcinoma cells in vitro and in vivo

Antitumor and antimetastatic activities of Rhizoma Paridis saponins

Thalidomide influences growth and vasculogenic mimicry channel formation in melanoma

Cyclophosphamide promotes pulmonary metastasis on mouse lung adenocarcinoma

Tanshinone IIA inhibits atherosclerotic plaque formation by down-regulating MMP-2 and MMP-9 expression in rabbits fed a high-fat diet

HAb18G/CD147 Functions in Invasion and Metastasis of Hepatocellular Carcinoma

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