Click image to see more details
Product Info Summary
| SKU: | A19781 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-MOBL3 Antibody
SKU/Catalog Number
A19781
Size
100ul
Form
Liquid
Description
Boster Bio Anti-MOBL3 Antibody (Catalog# A19781). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MOBL3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A19781)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human MOBL3. Immunogen sequence location: 1-50
Reactive Species
A19781 is reactive to MOB4 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
26.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A19781 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ICC/IF 1:200 - 1:1000.
ELISA: 1:20000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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Western blot analysis of the lysates from HT-29 cells using MOBL3 antibody.
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Western blot analysis of lysates from rat brain cells, using MOBL3 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-MOBL3 Antibody (A19781)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-MOBL3 Antibody
Question
We are currently using anti-MOBL3 antibody A19781 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-10
Answer
The anti-MOBL3 antibody (A19781) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-10
Question
Is a blocking peptide available for product anti-MOBL3 antibody (A19781)?
Verified Customer
Verified customer
Asked: 2020-03-20
Answer
We do provide the blocking peptide for product anti-MOBL3 antibody (A19781). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-03-20
Question
I see that the anti-MOBL3 antibody A19781 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-17
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-07-17
Question
Do you have a BSA free version of anti-MOBL3 antibody A19781 available?
Verified Customer
Verified customer
Asked: 2018-10-26
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MOBL3 antibody A19781 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-10-26
Question
See below the WB image, lot number and protocol we used for brain using anti-MOBL3 antibody A19781. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-04-16
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-04-16
Question
My question regarding product A19781, anti-MOBL3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
B. Zhao
Verified customer
Asked: 2016-11-23
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A19781 anti-MOBL3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-11-23
Question
Is this A19781 anti-MOBL3 antibody reactive to the isotypes of MOB4?
G. Mitchell
Verified customer
Asked: 2014-12-29
Answer
The immunogen of A19781 anti-MOBL3 antibody is A peptide derived from human MOBL3. Immunogen sequence location: 1-50. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-12-29
