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MOB1B / MOB kinase activator 1B · IHC design guide
Design Immunohistochemistry for MOB1B
Plan MOB1B staining in paraffin sections using the observed cytoplasmic tissue pattern (HPA tissue IHC). Compare positive glandular cells with adipocytes reported as not detected, while accounting for possible staining of more than one gene product (HPA tissue IHC).
Evidence assembled Oct 2026 · For research use; verify linked source records and product datasheet before use
MOB1B Immunohistochemistry Experimental Design Guide
Expected localisation, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before staining
| Expected localisation | Cytoplasmic tissue staining (HPA tissue IHC); nucleus annotated (UniProt) | |
| Staining pattern | Widespread cytoplasmic staining (HPA tissue IHC) | |
| Antigen retrieval | Tris-EDTA pH 9.0 HIER, heat-mediated (datasheet A09405-2) | |
| Positive control | Adrenal gland+4 more · see all | |
| Negative control | Adipose tissue+1 more · see all |
Important caveats
Reasons your staining may differ from the expected pattern.
| Fixation | Keep fixation consistent across sections. (standard IHC practice; not target-specific) | |
| Caveat | Staining may reflect more than one gene product (HPA tissue IHC) | |
| Regulation | No expression regulator specified (UniProt) | |
| Isoform / epitope | 2 isoforms; isoform-specific epitope coverage is unknown (UniProt) |
Section 1
Recommended MOB1B IHC & IF Protocols
The catalog antibody protocol uses Tris-EDTA pH 9.0 retrieval (datasheet A09405-2); the published IHC protocols below cover spinal cord and endometrial cancer tissue sections (PMC10877155; PMC10067393).
| Sample | Paraffin-embedded human tonsil tissue; fixative not specified (datasheet A09405-2) |
| Fixation | Image fixative and duration unreported (datasheet A09405-2); verify before use. |
| Sectioning | 4–5 µm sections on charged slides (standard) |
| Deparaffinisation | Xylene, graded ethanol series to water (standard) |
| Antigen retrieval | Heat retrieval: Tris-EDTA pH 9.0 (datasheet A09405-2); 20 min, 95–100 °C (standard) |
| Peroxidase block | 3% H2O2, 10 min, room temperature (standard) |
| Blocking | 10% normal serum of the secondary host, 30 min, room temperature (standard) |
| Primary antibody | Rabbit anti-MOB1B, 1:100-1:300 (datasheet A09405-2) |
| Primary incubation | Overnight at 4 °C (standard) |
| Detection | HRP-polymer secondary, DAB chromogen 5–10 min (standard) |
| Counterstain | Hematoxylin, blue, dehydrate and mount (standard) |
| Expected result | MOB1B-positive staining in glandular cells of adrenal gland (HPA tissue IHC: High). HPA tissue profile: Ubiquitous cytoplasmic expression. No signal in the no-primary control. |
💡Decision noteStart with heat-mediated Tris-EDTA pH 9.0 retrieval for the catalog antibody (datasheet A09405-2); the spinal cord paper reports microwaving without a retrieval buffer (PMC10877155).
Section 2
What Is the Expected MOB1B Staining Pattern?
In paraffin-section IHC, expect predominantly cytoplasmic staining in many cell types (HPA: ubiquitous cytoplasmic expression; tissue IHC Supported). Glandular cells in colon and adrenal gland and kidney tubular cells are useful high-staining examples (HPA: High). Nuclear localisation is also documented (UniProt Q7L9L4: Cytoplasm; Nucleus), while MOB1B has no transmembrane segment (UniProt Q7L9L4 topology). Interpret specificity cautiously: the HPA tissue profile carries a warning that antibodies may detect proteins from more than one gene (HPA: tissue IHC reliability description).
| Clear cytoplasmic staining in colon glandular cells or kidney tubular cells, with identifiable cell boundaries. | This fits the reported IHC distribution and offers an internal reference for evaluating weaker cells on the same section (HPA: High in colon glandular cells and kidney tubular cells; ubiquitous cytoplasmic expression). It supports a plausible pattern, but staining alone cannot prove that the antibody distinguishes MOB1B from related proteins (HPA: tissue IHC multi-gene caution). |
| Staining is confined to plasma membranes or extracellular material, with little cellular staining. | A membrane-only or extracellular pattern conflicts with the reported cellular locations and warrants an artefact check (HPA: ubiquitous cytoplasmic IHC expression; UniProt Q7L9L4: Cytoplasm; Nucleus; no transmembrane segment). Nuclear staining alone is a less decisive warning because nuclear localisation is documented; compare it with cytoplasmic signal and controls before judging the section. |
| Adipocytes or peripheral nerves stain strongly while nearby expected positive cells do not. | That distribution conflicts with the reported tissue examples (HPA: Not detected in adipocytes and soft-tissue peripheral nerve; High in colon glandular cells). Consider cross-reactivity, section background, or endogenous detection activity as possibilities. These HPA negatives are comparisons for interpretation, not a guarantee that every specimen or assay will be blank. |
| Diffuse colour covers stroma, empty spaces, and cells without a readable cytoplasmic pattern. | Treat the result as uninterpretable until background is controlled; diffuse deposit does not reproduce the reported cellular IHC pattern (HPA: ubiquitous cytoplasmic expression). As a general chromogenic IHC check, compare the no-primary control and inspect blocking, washing, and detection conditions. Those checks are workflow advice, not evidence of a MOB1B-specific background mechanism. |
| A known positive area shows no cellular signal, including colon glands or kidney tubules. | A blank result in those compartments conflicts with reported high staining and may indicate an assay or section problem (HPA: High in colon glandular cells and kidney tubular cells). Check the assay's positive control before calling the specimen negative. The HPA tissue profile does not identify a MOB1B-specific fixation sensitivity or establish why a particular section failed. |
💡Expected MOB1B appearanceCall a section plausibly positive when glandular or tubular cells show distinct, predominantly cytoplasmic signal of appreciable intensity (HPA: High in colon glandular cells and kidney tubular cells; ubiquitous cytoplasmic expression); membrane-only or diffuse extracellular colour is a warning pattern (UniProt Q7L9L4 topology; HPA: tissue IHC profile).
| Compartment and application | For paraffin IHC, use the reported cytoplasmic tissue pattern as the primary comparison (HPA: ubiquitous cytoplasmic expression). For the separate IF/ICC question—can nuclear signal be plausible?—yes: nucleoplasm and nucleoli are supported main locations, with additional cytosol (HPA: subcellular ICC-IF); UniProt also lists Nucleus and Cytoplasm (UniProt Q7L9L4). This IF observation does not supply an IHC protocol option. |
| Cell context | High staining in colon and adrenal glandular cells and kidney tubular cells provides practical IHC reference areas (HPA: High). Adipocytes and soft-tissue peripheral nerves were not detected in the supplied profile (HPA: Not detected). Compare the named cell populations, since a whole-section positive or negative label can hide a mismatch in the cells carrying the signal. |
| Antibody interpretation | The tissue IHC profile is Supported, with medium agreement between staining and RNA expression and a warning about proteins from multiple genes (HPA: reliability description). The listed IHC antibody HPA047088 is Supported; HPA071690 has an ICC approval entry but no supplied IHC status (HPA: antibody validation). These designations support pattern review without establishing absolute MOB1B specificity. |
| Situation | Likely cause | Next action |
|---|---|---|
| Colon glands or kidney tubules are blank. | The observed section disagrees with the supplied high-staining examples; the evidence does not identify a target-specific failure mechanism (HPA: High in colon glandular cells and kidney tubular cells). | Check a positive-control section and the primary-antibody, detection, and counterstain steps as general IHC practice. Record whether the relevant cell type is present and preserved before reporting a negative result; do not assign the failure to MOB1B fixation sensitivity without separate evidence. |
| Only membranes or extracellular spaces carry colour. | That distribution is inconsistent with the reported intracellular locations and absence of a transmembrane segment (HPA: ubiquitous cytoplasmic IHC expression; UniProt Q7L9L4 topology). | Compare the same cell type in a positive reference area, inspect section morphology, and review the no-primary control. Reassess the staining as possible artefact if cellular cytoplasmic signal cannot be demonstrated; membrane colour alone does not match the supplied reference pattern. |
| Adipocytes or peripheral nerves are prominent positives. | These cells were not detected in the supplied HPA tissue examples, so cross-reactivity or endogenous detection activity is worth checking (HPA: Not detected in adipocytes and soft-tissue peripheral nerve). | Check the no-primary control for detection background and compare a named high-staining cell population on a control section (HPA: High in colon glandular cells). Interpret any remaining unexpected signal cautiously because the tissue profile warns of possible detection of proteins from more than one gene (HPA: reliability description). |
| Diffuse chromogen obscures cell boundaries. | The deposit prevents a reliable comparison with the reported cytoplasmic pattern (HPA: ubiquitous cytoplasmic expression). Its cause cannot be assigned from the target evidence alone. | As general IHC practice, inspect no-primary background, blocking, washes, and detection exposure, then repeat with conditions that preserve readable morphology. Score the cellular pattern only after diffuse colour is reduced; the supplied HPA data do not identify a MOB1B-specific background source. |
| A nucleus-heavy IHC result appears inconsistent with the expected cytoplasmic tissue pattern. | The sources describe different application-level observations: cytoplasmic tissue IHC and supported nucleoplasm, nucleoli, and cytosol in ICC-IF (HPA: tissue IHC profile; HPA: subcellular ICC-IF). UniProt also lists both Nucleus and Cytoplasm (UniProt Q7L9L4). | Do not reject nuclear signal solely by compartment. Check whether the named IHC-positive cell types also show a credible cellular pattern and whether controls are clean (HPA: High in colon glandular cells and kidney tubular cells). Use the separate IF/ICC guide for IF methods. |
| Signal is present, but its assignment specifically to MOB1B matters. | The supplied HPA tissue assessment warns that the staining may represent proteins from more than one gene despite its Supported rating (HPA: tissue IHC reliability description). | Report the observed cell type and compartment with that specificity caveat. If a MOB1B-specific conclusion is required, seek independent validation suitable for the research question; the supplied IHC pattern and antibody status alone do not settle gene-level specificity (HPA: tissue IHC reliability description; HPA: antibody validation). |
Experiment evidence
IHC & IF images showing MOB1B staining
A09405-2 · IHCImmunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH9.0 was use
A30515 · IHCImmunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used
A09405-3 · IHCFormalin-fixed and paraffin-embedded human brain tissue reacted with MOB4A antibody (C-term) , which was peroxidase-conjugated to the second
A09405-3 · IFFluorescent image of NIH/3T3 cell stained with MOB4A Antibody (C-term).NIH/3T3 cells were fixed with 4% PFA (20 min), permeabilized with Tri
HPA · Cerebral cortexHuman Protein Atlas IHC, Cerebral cortex. Source: https://www.proteinatlas.org/ENSG00000173542-MOB1B/tissue/cerebral+cortex#imid_15864567
HPA · Adrenal glandHuman Protein Atlas IHC, Adrenal gland. Source: https://www.proteinatlas.org/ENSG00000173542-MOB1B/tissue/adrenal+gland#imid_15864543
HPA · ColonHuman Protein Atlas IHC, Colon. Source: https://www.proteinatlas.org/ENSG00000173542-MOB1B/tissue/colon#imid_15864501
HPA · LiverHuman Protein Atlas IHC, Liver. Source: https://www.proteinatlas.org/ENSG00000173542-MOB1B/tissue/liver#imid_15864510
HPA · ICC-IF RT-4Human Protein Atlas immunofluorescence, RT-4, antibody HPA071690; green = MOB1B, blue = nucleus, red = microtubules.
HPA · ICC-IF RT-4Human Protein Atlas immunofluorescence, RT-4, antibody HPA071690; green = MOB1B, blue = nucleus, red = microtubules.
← swipe / scroll for more images →
Real IHC / IF data behind the staining-pattern expectations in this section.
Sample controls for MOB1B IHC & IF
🧪Run adrenal gland first: its glandular cells should stain (HPA: High in adrenal gland glandular cells). Use adipose tissue as the negative comparator (HPA: Not detected in adipocytes); on the adrenal slide, cells without specific staining should remain at background, but their MOB1B-negative status is unconfirmed.
Positive control tissue: Adrenal gland (Glandular cells, HPA High)
Negative control tissue: Adipose tissue (HPA Not detected)
ICC-IF cell lines (HPA subcellular resource): HPA ICC-IF images show MOB1B in RT-4, Rh30, U2OS, with annotated localisation: Nucleoplasm (supported), Nucleoli (supported) (HPA subcellular).
Technical controls: Include a no-primary (secondary-only) control, an isotype control matched to the primary antibody’s host species and clonality, and a biological specificity control using MOB1B knockout tissue or cognate-peptide competition if available. Quench endogenous peroxidase for chromogenic IHC and block endogenous biotin if using biotin-based detection (standard IHC practice).
⚠️Feasibility: A target-specific fixation window or fixation effect is unreported, and the selected A09405-2 paraffin-section caption does not state a fixative (datasheet: fixative unreported). The caption uses Tris-EDTA pH 9.0 retrieval in tonsil, so retrieval conditions for adrenal gland need optimization (datasheet: tonsil retrieval); the supplied evidence does not establish that frozen sections or IF are easier. For IF, interpret nuclear and cytosolic signal cautiously because the HPA localization images carry a multiple-gene antibody warning (HPA: subcellular caution).
HPA tissue IHC evidence for MOB1B
Comprehensive Human Protein Atlas IHC scoring per tissue (reliability: Supported — Medium consistency between antibody staining and RNA expression data. Caution, targets protein from more than one gene.). Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adrenal gland | Glandular cells | High | Protein (IHC) | HPA → |
| Appendix | Glandular cells | High | Protein (IHC) | HPA → |
| Cerebellum | Cells in granular layer | High | Protein (IHC) | HPA → |
| Colon | Glandular cells | High | Protein (IHC) | HPA → |
| Duodenum | Glandular cells | High | Protein (IHC) | HPA → |
Section 3
Advanced MOB1B IHC Tips
Troubleshoot MOB1B staining in paraffin section IHC by checking retrieval, tissue handling, cellular localisation and detection controls before comparing specimens.
Which antigen retrieval should I use when MOB1B staining is weak?
Start with heat mediated retrieval in Tris-EDTA at pH 9.0 for paraffin sections (datasheet A09405-2). The selected tonsil image used that buffer, a 1:200 primary overnight at 4°C, and a 1:200 secondary for 45 min at room temperature (caption A09405-2). If staining is weak, verify deparaffinisation and heating consistency, then vary retrieval time while keeping the buffer and pH fixed; compare serial sections with identical detection conditions (standard IHC practice). Judge any signal gain alongside morphology and background, because excessive heating can damage sections and increase nonspecific staining (standard IHC practice).
How should I handle fixation when MOB1B staining varies between paraffin specimens?
Target-specific sensitivity to fixative choice or duration is unknown for MOB1B; the selected paraffin tonsil caption does not report its fixative (caption A09405-2). Record each specimen’s fixative, fixation duration, processing schedule and section age before comparing staining, and keep those variables consistent where possible (standard IHC practice). Assess morphology and antigen preservation with a known positive control in each run, because differences in tissue handling can complicate comparisons (standard IHC practice). Start with Tris-EDTA at pH 9.0 and the 1:200 primary condition, then change one workflow variable at a time on matched sections (caption A09405-2; standard IHC practice).
Should I expect MOB1B signal in the cytoplasm or nucleus?
UniProt lists MOB1B in the cytoplasm and nucleus, while HPA describes tissue IHC staining as ubiquitously cytoplasmic (UniProt Q7L9L4; HPA tissue IHC). Score cytoplasmic and nuclear staining separately in identifiable cells, using the counterstain to define nuclear boundaries (standard IHC practice). HPA subcellular IF reports nucleoplasm, nucleoli and cytosol, but cautions that its antibodies may target proteins from more than one gene (HPA subcellular). If an unexpected compartment dominates, inspect control sections, tissue edges and detection background before calling a change in localisation; independently verify specificity when that claim is central to the experiment (HPA subcellular; standard IHC practice).
Can this IHC stain distinguish MOB1B isoforms or phosphorylation states?
MOB1B has 2 annotated isoforms, but the supplied product caption does not identify the antibody epitope or establish isoform selectivity (UniProt Q7L9L4; caption A09405-2). UniProt lists phosphorylation by STK4/MST1 at threonines 12 and 35; ordinary total protein IHC cannot assign staining to either modification without a validated modification-specific reagent (UniProt Q7L9L4; standard IHC practice). Check the antibody’s epitope documentation before comparing isoforms, and use an independently validated assay if isoform identity determines the conclusion (standard IHC practice). Keep retrieval at Tris-EDTA pH 9.0 during that comparison so preparation differences do not obscure an epitope question (caption A09405-2; standard IHC practice).
How can IF help check the cellular source of a MOB1B IHC signal?
Use IF as a separate localisation check, multiplexing MOB1B with a validated marker for the cell population scored in the IHC section, such as the relevant glandular or tubular cells (HPA tissue IHC; standard IF practice). Choose well separated fluorophores and consider a far red channel where tissue autofluorescence interferes with shorter wavelength signals (standard IF practice). MOB1B has no annotated transmembrane segment and is reported in cytoplasm and nucleus, so use permeabilisation appropriate for access to intracellular epitopes, with a matched control for background (UniProt Q7L9L4; standard IF practice). HPA IF shows nucleoplasm, nucleoli and cytosol but carries a multiple gene targeting caution; do not assume its pattern proves this antibody’s specificity (HPA subcellular).
How do I reduce diffuse brown staining without losing MOB1B signal?
First compare a primary omission control with a known positive section to distinguish detection background from loss of the intended stain (standard IHC practice). For chromogenic detection, block endogenous peroxidase, use an appropriate protein block, wash consistently and control DAB development under the same conditions across sections (standard IHC practice). The tonsil example used a 1:200 primary overnight at 4°C and a 1:200 secondary for 45 min; titrate either reagent if diffuse colour persists (caption A09405-2; standard IHC practice). Preserve the expected cytoplasmic pattern while assessing background, and inspect edges and damaged regions separately from intact tissue (HPA tissue IHC; standard IHC practice).
What scoring method makes MOB1B IHC comparisons reproducible? ⚠ ANSWER MARKED FOR VERIFICATION
Define the cell population and compartment before scoring, because HPA reports broadly cytoplasmic tissue staining while UniProt also lists a nuclear location (HPA tissue IHC; UniProt Q7L9L4). For intact identified cells, record the percentage positive and staining intensity, then calculate an H-score using percentages at intensity grades 0–3 if that matches the study question (standard IHC practice). Alternatively, report positive cell density per mm² when cell abundance itself is the outcome, using the same region selection and threshold across samples (standard IHC practice). Normalise to the number of eligible cells or analysed tissue area, and score nuclear and cytoplasmic signals separately where relevant (standard IHC practice).
How can I tell a genuine MOB1B positive from staining artefact?
Look for reproducible staining in intact, identifiable cells and a plausible cytoplasmic or nuclear distribution; UniProt lists both compartments, and HPA describes cytoplasmic tissue staining (UniProt Q7L9L4; HPA tissue IHC). HPA reports high staining in kidney tubule cells and several glandular populations, while adipocytes were not detected, but its tissue assessment has a multiple gene targeting caution (HPA tissue IHC). Treat colour concentrated at section edges, necrotic areas or endogenous enzyme rich regions as suspect until primary omission and peroxidase block controls are reviewed (standard IHC practice). A clean control and matching morphology support interpretation, but an important MOB1B-specific claim still needs independent specificity evidence (HPA tissue IHC; standard IHC practice).
Boster reagents
Best MOB1B / MOB kinase activator 1B IHC Antibodies
The catalog lists MOB1B-labeled antibodies with human tonsil IHC images and human, monkey, and mouse reactivity; IF is listed as an application (catalog: A09405-2, A30515).
Real IHC data
Anti-Mob1A MOB1B Antibody
Cat # A09405-2
Real IHC data
Anti-MOL1A MOB1B Antibody
Cat # A30515
Real IHC data
Anti-MOB4A Antibody (C-term)
Cat # A09405-3
A09405-2 and A30515 each show IHC on paraffin-embedded human tonsil; neither caption reports the fixative (catalog: A09405-2 and A30515 IHC captions). A09405-3 shows IHC on formalin-fixed, paraffin-embedded human brain and IF in NIH/3T3 cells, but its catalog target is MOB4A (catalog: A09405-3 title and image captions).
Which to pick: For MOB1B tissue IHC, choose A09405-2 or A30515: both are rabbit polyclonals with IHC listed and their own paraffin-embedded human tonsil images; fixation is unreported in both captions (catalog: A09405-2 and A30515 application lists, dilution data, and IHC captions). For IF or ICC, A30515 lists both applications, while A09405-2 lists IF only; neither has an IF image in this payload (catalog: A30515 and A09405-2 application lists and image records). For human, monkey, or mouse samples, both list reactivity across those species; A09405-3 targets MOB4A and should not be selected to measure MOB1B (catalog: reactivity lists and A09405-3 title).
Each figure is that product's own IHC / IF validation image from its datasheet.
References
- UniProt Consortium. UniProt entry Q7L9L4 (MOB1B_HUMAN, MOB kinase activator 1B).
- Human Protein Atlas. MOB1B tissue IHC expression (reliability: Supported).
- Human Protein Atlas. MOB1B subcellular location (ICC-IF): Mainly localized to the nucleoplasm and nucleoli. In addition localized to the cytosol. Caution: Based on antibodies targeting proteins from multiple genes..
- Human Protein Atlas. MOB1B antibody validation summary (2 antibodies).
- The compensatory increase of Gli-similar 3 inhibited neuronal apoptosis through regulating Mps one binder kinase activator 1b (MOB1b): a possible strategy for the functional recovery after spinal cord injury. Experimental animals 2024 — PMC10877155.
- miR-548ag functions as an oncogene by suppressing MOB1B in the development of obesity-related endometrial cancer. Cancer science 2023 — PMC10067393.
- KIAA1429-mediated m6A modification of CHST11 promotes progression of diffuse large B-cell lymphoma by regulating Hippo-YAP pathway. Cellular & molecular biology letters 2023 — PMC10114474.
- PubMed PMID:14702039 — UniProt-cited evidence.
- PubMed PMID:15489334 — UniProt-cited evidence.
- PubMed PMID:15067004 — UniProt-cited evidence.
Immunohistochemistry protocol sheet for MOB1B (MOB kinase activator 1B). Expected localisation Cytoplasmic tissue staining (HPA tissue IHC); nucleus annotated (UniProt). Antigen retrieval: Tris-EDTA pH 9.0 HIER, heat-mediated (datasheet A09405-2). Validated IHC antibody: A09405-2 at 1:100-1:300 (the product's own IHC validation image is shown). Controls (HPA): positive Adrenal gland (Glandular cells); negative Adipose tissue. IHC workflow; specimen preparation unspecified. Protocol steps: 1. Sample: Paraffin-embedded human tonsil tissue; fixative not specified (datasheet A09405-2); 2. Fixation: Image fixative and duration unreported (datasheet A09405-2); verify before use.; 3. Sectioning: 4–5 µm sections on charged slides (standard); 4. Deparaffinisation: Xylene, graded ethanol series to water (standard); 5. Antigen retrieval: Heat retrieval: Tris-EDTA pH 9.0 (datasheet A09405-2); 20 min, 95–100 °C (standard); 6. Peroxidase block: 3% H2O2, 10 min , room temperature (standard); 7. Blocking: 10% normal serum of the secondary host, 30 min , room temperature (standard); 8. Primary antibody: Rabbit anti-MOB1B, 1:100-1:300 (datasheet A09405-2); 9. Primary incubation: Overnight at 4 °C (standard). Watch for: Fixation: Keep fixation consistent across sections. (standard IHC practice; not target-specific); Caveat: Staining may reflect more than one gene product (HPA tissue IHC); Isoform / epitope: 2 isoforms; isoform-specific epitope coverage is unknown (UniProt); Regulation: No expression regulator specified (UniProt). Evidence: UniProt Q7L9L4, HPA tissue IHC · Supported, HPA ICC-IF · Mainly localized to the nucleoplasm and nucleoli. In addition…, Datasheet A09405-2, PMC10877155, PMC10067393. QR code links to https://www.bosterbio.com/bosterbio-gene-info-cards/MOB1B/immunohistochemistry-ihc-guide.