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- Table of Contents
Dnmt1 antibody (M00172-2) performs well in IF, IHC-P, WB and shows reactivity with Human, Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Dnmt1 with M00172-2 at 1/50 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red).
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Anti-(Mouse) Dnmt1 Antibody (Center)
Dnmt1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse | Rabbit | IF, IHC-P, WB |
Recommend Dilution
Application
Dilution
Species
IF: 1:50
WB: 1:1000
IHC-P: 1:25
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Product Information
Product Name
Anti-(Mouse) Dnmt1 Antibody (Center)
View all DNMT1 Antibodies
View all DNMT1 Antibodies
Uniprot
Gene ID
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This MouseDnmt1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 881-914 amino acids from the Central region of mouse Dnmt1.
Calculated Molecular Weight
183.2 kDa
Background of Dnmt1
Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9.
Cite This Product
Anti-(Mouse) Dnmt1 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00172-2)
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Product Promise
We promise Anti-(Mouse) Dnmt1 Antibody (Center) performs excellently in relevant applications for IF, IHC-P, WB. If it doesn't perform as promised, we will refund the product. Learn more
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M00172-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 12, 2026