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Product Info Summary
| SKU: | M00276-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-(Mouse) Epcam Antibody (C-term)
SKU/Catalog Number
M00276-1
Size
80 µl
Description
Boster Bio Anti-(Mouse) Epcam Antibody (C-term) (Catalog # M00276-1). Tested in WB, IHC-P application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-(Mouse) Epcam Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00276-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This Mouse Epcam antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 217-251 amino acids from the C-terminal region of Mouse Epcam.
Reactive Species
M00276-1 is reactive to Epcam in Human, Mouse, Rat
Calculated molecular weight
35.0 kDa
Background of Epcam
May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E (By similarity).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00276-1 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC-P: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-Epcam Antibody (C-term) at 1:2000 dilution
Lane 1: mouse kidney lysates
Lane 2: mouse colon lysates
Lane 3: MCF-7 whole cell lysates
Lane 4: rat colon lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 35 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Epcam Antibody (C-term) at 1:2000 dilution
Lane 1: mouse lung lysates
Lane 2: mouse kidney lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 35 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Click image to see more details
M00276-1 staining (Mouse) Epcam in mouse colon sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Specific Publications For Anti-(Mouse) Epcam Antibody (C-term) (M00276-1)
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