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Product Info Summary
| SKU: | M03568-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-(Mouse) Med15 Antibody (C-term)
SKU/Catalog Number
M03568-1
Size
80 µl
Description
Boster Bio Anti-(Mouse) Med15 Antibody (C-term) (Catalog # M03568-1). Tested in WB, IHC-P application(s). This antibody reacts with Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-(Mouse) Med15 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03568-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This mouse Med15 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 712-746 amino acids from the C-terminal region of mouse Med15.
Reactive Species
M03568-1 is reactive to Med15 in Mouse, Rat
Calculated molecular weight
86.6 kDa
Background of Med15
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for cholesterol-dependent gene regulation. Positively regulates the Nodal signaling pathway (By similarity).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03568-1 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC-P: 1:25
Validation Images & Assay Conditions
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Anti-Med15 Antibody (C-term) at 1:1000 dilution + mouse testis lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 87 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Med15 Antibody (C-term) at 1:2000 dilution
Lane 1: mouse brain lysates
Lane 2: mouse spleen lysates
Lane 3: mouse thymus lysates
Lane 4: rat lung lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 87 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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M03568-1 staining (Mouse) Med15 in Mouse heart tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Specific Publications For Anti-(Mouse) Med15 Antibody (C-term) (M03568-1)
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