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Product Info Summary
| SKU: | M06772-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-(Mouse) Mlf1 Antibody (Center)
SKU/Catalog Number
M06772-1
Size
80 µl
Description
Boster Bio Anti-(Mouse) Mlf1 Antibody (Center) (Catalog # M06772-1). Tested in WB application(s). This antibody reacts with Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-(Mouse) Mlf1 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06772-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This Mouse Mlf1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 91-126 amino acids from the Central region of Mouse Mlf1.
Reactive Species
M06772-1 is reactive to Mlf1 in Mouse
Calculated molecular weight
30.4 kDa
Background of Mlf1
Involved in lineage commitment of primary hemopoietic progenitors by restricting erythroid formation and enhancing myeloid formation. Interferes with erythropoietin-induced erythroid terminal differentiation by preventing cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. Suppresses RFWD2/COP1 activity via CSN3 which activates p53 and induces cell cycle arrest. Binds DNA and affects the expression of a number of genes so may function as a transcription factor in the nucleus.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06772-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500
Validation Images & Assay Conditions
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Anti-Mlf1 Antibody (Center)at 1:500 dilution + mouse testis lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 30 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Specific Publications For Anti-(Mouse) Mlf1 Antibody (Center) (M06772-1)
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