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Product Info Summary
| SKU: | A03105 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-(Mouse) Tdgf1 Antibody (N-term)
SKU/Catalog Number
A03105
Size
80 µl
Description
Boster Bio Anti-(Mouse) Tdgf1 Antibody (N-term) (Catalog # A03105). Tested in WB, Flow Cytometry application(s). This antibody reacts with Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-(Mouse) Tdgf1 Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03105)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This Mouse Tdgf1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 37-70 amino acids from the N-terminal region of mouse Tdgf1.
Reactive Species
A03105 is reactive to Tdgf1 in Mouse
Calculated molecular weight
18.8 kDa
Background of Tdgf1
Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03105 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
FC: 1:25
Validation Images & Assay Conditions
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Western blot analysis of lysate from mouse F9 cell line, using (Mouse) Tdgf1 Antibody (N-term). A03105 was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
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Overlay histogram showing F9 cells stained with A03105 (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (A03105, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit lgG (H+L) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Specific Publications For Anti-(Mouse) Tdgf1 Antibody (N-term) (A03105)
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Customer Q&As
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6 Customer Q&As for Anti-(Mouse) Tdgf1 Antibody (N-term)
Question
Is a blocking peptide available for product anti-CRIPTO antibody (A03105)?
Verified Customer
Verified customer
Asked: 2020-03-02
Answer
We do provide the blocking peptide for product anti-CRIPTO antibody (A03105). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-03-02
Question
Here is the WB image, lot number and protocol we used for cortex of kidney using anti-CRIPTO antibody A03105. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-22
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cortex of kidney using anti-CRIPTO antibody A03105. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-14
Question
Is this A03105 anti-CRIPTO antibody reactive to the isotypes of TDGF1?
Verified Customer
Verified customer
Asked: 2019-05-10
Answer
The immunogen of A03105 anti-CRIPTO antibody is Synthetic peptide with sequence YGRNCEHDVRKEN, corresponding to the internal region of the protein sequence. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-10
Question
I was wanting to use your anti-CRIPTO antibody for WB for rat cortex of kidney on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat cortex of kidney identification?
V. Mitchell
Verified customer
Asked: 2019-01-01
Answer
As indicated on the product datasheet, A03105 anti-CRIPTO antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat cortex of kidney in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-01-01
Question
We are currently using anti-CRIPTO antibody A03105 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2018-07-24
Answer
The anti-CRIPTO antibody (A03105) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-07-24
