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Product Info Summary
| SKU: | A08340 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-MPP1/KIF20B Antibody Picoband®
SKU/Catalog Number
A08340
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MPP1/KIF20B Antibody Picoband® catalog # A08340. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MPP1/KIF20B Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08340)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human MPP1/KIF20B recombinant protein (Position: A19-D231).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A08340 is reactive to KIF20B in Human, Mouse, Rat
Observed Molecular Weight
211 kDa
Calculated molecular weight
210.6 kDa
Background of KIF20B
Kinesin-like protein KIF20B is a protein that in humans is encoded by the KIF20B gene. The MPP1 gene is previously labeled as KIF20B. Palmitoylated membrane protein 1 is the prototype of a family of membrane-associated proteins termed MAGUKs (membrane-associated guanylate kinase homologs). MAGUKs interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intracellular junctions. Palmitoylated membrane protein 1 contains a conserved sequence, called the SH3 (src homology 3) motif, found in several other proteins that associate with the cytoskeleton and are suspected to play important roles in signal transduction.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08340 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human PC-3 whole cell, human A549 whole cell
Validation Images & Assay Conditions
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Boster Kit Box
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Western blot analysis of KIF20B using anti-KIF20B antibody (A08340).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates,
Lane 2: human A549 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KIF20B antigen affinity purified polyclonal antibody (Catalog # A08340) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KIF20B at approximately 211KD. The expected band size for KIF20B is at 211KD.
Specific Publications For Anti-MPP1/KIF20B Antibody Picoband® (A08340)
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1 Customer Q&As for Anti-MPP1/KIF20B Antibody Picoband®
Question
We are currently using anti-MPP1/KIF20B antibody A08340 for mouse tissue, and we are satisfied with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2017-05-29
Answer
The anti-MPP1/KIF20B antibody (A08340) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-05-29
