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Product Info Summary
| SKU: | A07143 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-MPP7 Antibody (N-term)
SKU/Catalog Number
A07143
Size
80 µl
Description
Boster Bio Anti-MPP7 Antibody (N-term) (Catalog # A07143). Tested in WB, IHC-P, Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-MPP7 Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07143)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This MPP7 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 2-36 amino acids from the N-terminal region of human MPP7.
Reactive Species
A07143 is reactive to MPP7 in Human
Calculated molecular weight
65.5 kDa
Background of MPP7
Acts as an important adapter that promotes epithelial cell polarity and tight junction formation via its interaction with DLG1. Involved in the assembly of protein complexes at sites of cell-cell contact.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07143 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:25
FC: 1:25
Validation Images & Assay Conditions
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Western blot analysis of lysate from human ovary tissue lysate, using MPP7 Antibody (N-term) . A07143 was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
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Immunohistochemical analysis of paraffin-embedded H. kidney section using MPP7 Antibody (N-term) (Cat#A07143). A07143 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Immunohistochemical analysis of paraffin-embedded H. liver section using MPP7 Antibody (N-term) (Cat#A07143). A07143 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Immunohistochemical analysis of paraffin-embedded M. kidney section using MPP7 Antibody (N-term) (Cat#A07143). A07143 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Flow cytometric analysis of Hela cells using MPP7 Antibody (N-term) (green, Cat#A07143) compared to an isotype control of rabbit IgG (blue). A07143 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Specific Publications For Anti-MPP7 Antibody (N-term) (A07143)
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