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- Table of Contents
ABCC1 antibody (A00872-1) performs well in ELISA, Flow Cytometry, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 7 Publications
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Flow Cytometry analysis of SiHa cells using anti-MRP1/ABCC1 antibody (A00872-1).
Overlay histogram showing SiHa cells stained with A00872-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-MRP1/ABCC1 Antibody (A00872-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing SiHa cells stained with A00872-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-MRP1/ABCC1 Antibody (A00872-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-MRP1/ABCC1 Antibody Picoband®
ABCC1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | ELISA, Flow Cytometry, WB |
Tested Applications
ABCC1 antibody was positively detected by WB in:
human PANC-1 whole cell, human A549 whole cell, human HepG2 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
ABCC1 antibody was positively detected by FC in:
SiHa cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
ELISA
0.1-0.5 μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
ELISA
Human
Tested
Recommend
Monkey
Predicted
Predicted
Product Information
Product Name
Anti-MRP1/ABCC1 Antibody Picoband®
View all ABCC1 Antibodies
View all ABCC1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human MRP1/ABCC1 recombinant protein (Position: S239-K357).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
220 kDa
Calculated Molecular Weight
171.6 kDa
Background of ABCC1
Multidrug resistance-associated protein 1 (MRP1) is a protein that in humans is encoded by the ABCC1 gene. The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra-and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This full transporter is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions as a multispecific organic anion transporter, with oxidized glutatione, cysteinyl leukotrienes, and activated aflatoxin B1 as substrates. This protein also transports glucuronides and sulfate conjugates of steroid hormones and bile salts. Alternatively spliced variants of this gene have been described but their full-length nature is unknown.
Cite This Product
Anti-MRP1/ABCC1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00872-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
97%
High Homology
Cow
87%
Moderate Homology
Sheep
86%
Moderate Homology
Rabbit
82%
Moderate Homology
Dog
80%
Moderate Homology
Mouse
76%
Moderate Homology
Rat
76%
Moderate Homology
Zebrafish
52%
Low homology
Drosophila
29%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for ABCC1 antibody
Flow Cytometry (FC) protocol for ABCC1 antibody
Publications
Application:
Species:
E35 ablates acute leukemia stem and progenitor cells in vitro and in vivo
Published: 2020-01-21
Journal: JOURNAL OF CELLULAR PHYSIOLOGY
Application: WB
Impact Factor: 5.55
Species: Human
PMID: 31960417
Differential expression and response to arsenic stress of MRPs and ASAN1 determine sensitivity of classical multidrug-resistant leukemia cells to arsenic trioxide
Published: 2016-10-03
Journal: LEUKEMIA RESEARCH
Application: WB
Impact Factor: 2.61
Species: Human
PMID: 27736728
Cisplatin-induced CCL5 secretion from CAFs promotes cisplatin-resistance in ovarian cancer via regulation of the STAT3 and PI3K/Akt signaling pathways
Published: 2016-03-15
Journal: INTERNATIONAL JOURNAL OF ONCOLOGY
Application: WB
Impact Factor: 3.02
Species: Human
PMID: 26983899
Mechanistic Analysis of Taxol-induced Multidrug Resistance in an Ovarian Cancer Cell Line
Published: 2013-09-30
Journal: Asian Pacific Journal of Cancer Prevention
Application: WB
Impact Factor: 1.27
Species: Human
PMID: 24175763
Vascular endothelial growth factor induces multidrug resistance-associated protein 1 overexpression through phosphatidylinositol-3-kinase /protein kinase B signaling pathway and transcription factor specificity protein 1 in BGC823 cell line
Published: 2013-06-13
Journal: ACTA BIOCHIMICA ET BIOPHYSICA SINICA
Application: WB
Impact Factor: 1.81
Species: Human
PMID: 23765166
Shugoshin1 enhances multidrug resistance of gastric cancer cells by regulating MRP1, Bcl-2, and Bax genes
Published: 2013-04-06
Journal: TUMOR BIOLOGY
Application: WB
Impact Factor: 2.52
Species: Human
PMID: 23564482
Reversion of multidrug resistance by co-encapsulation of doxorubicin and curcumin in chitosan/poly(butyl cyanoacrylate) nanoparticles
Published: 2012-01-17
Journal: INTERNATIONAL JOURNAL OF PHARMACEUTICS
Application: WB
Impact Factor: 3.35
Species: Human
PMID: 22274587
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Product Promise
We promise Anti-MRP1/ABCC1 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A00872-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 13, 2026