Product Info Summary
SKU: | A13011 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-MRPL14 Antibody
SKU/Catalog Number
A13011
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-MRPL14 Antibody (Catalog # A13011). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MRPL14 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13011)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human MRPL14.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A13011 is reactive to MRPL14 in Human, Mouse, Rat
Applications
A13011 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
15.948kDa
Background of MRPL14
Forms part of 2 intersubunit bridges in the assembled ribosome. Upon binding to MALSU1 intersubunit bridge formation is blocked, preventing ribosome formation and repressing translation Probable.
Mungall A.J., Nature 425:805-811(2003).
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Kenmochi N., Genomics 77:65-70(2001).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from HeLa cells, COLO cells and 293 cells, using MRPL14 antibody A13011.
Protein Target Info & Infographic
Gene/Protein Information For MRPL14 (Source: Uniprot.org, NCBI)
Gene Name
MRPL14
Full Name
39S ribosomal protein L14, mitochondrial
Weight
15.948kDa
Superfamily
universal ribosomal protein uL14 family
Alternative Names
L14mt; L32mt; mitochondrial ribosomal protein L14; MRPL32MGC70566,39S ribosomal protein L32, mitochondrial; MRP-L32MRP-L14; RMPL32; RPML3239S ribosomal protein L14, mitochondrial MRPL14 L14mt, L32mt, MRP-L14, MRP-L32, MRPL32, RMPL32, RPML32 mitochondrial ribosomal protein L14 39S ribosomal protein L14, mitochondrial|39S ribosomal protein L32, mitochondrial|mitochondrial large ribosomal subunit protein uL14m
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MRPL14, check out the MRPL14 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MRPL14: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MRPL14 Antibody (A13011)
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No publications found for A13011
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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7 Customer Q&As for Anti-MRPL14 Antibody
Question
I have attached the WB image, lot number and protocol we used for quadriceps femoris using anti-MRPL14 antibody A13011. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-03-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-17
Question
Is this A13011 anti-MRPL14 antibody reactive to the isotypes of MRPL14?
Verified Customer
Verified customer
Asked: 2019-09-09
Answer
The immunogen of A13011 anti-MRPL14 antibody is Synthesized peptide derived from internal of human MRPL14. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-09
Question
I have a question about product A13011, anti-MRPL14 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-11-08
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A13011 anti-MRPL14 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-11-08
Question
We are currently using anti-MRPL14 antibody A13011 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?
V. Moore
Verified customer
Asked: 2018-06-28
Answer
The anti-MRPL14 antibody (A13011) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-06-28
Question
I see that the anti-MRPL14 antibody A13011 works with WB, what is the protocol used to produce the result images on the product page?
D. Roberts
Verified customer
Asked: 2017-10-25
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-10-25
Question
I would like to test anti-MRPL14 antibody A13011 on rat quadriceps femoris for research purposes, then I may be interested in using anti-MRPL14 antibody A13011 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-09-11
Answer
The products we sell, including anti-MRPL14 antibody A13011, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-09-11
Question
Will A13011 anti-MRPL14 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
J. Wu
Verified customer
Asked: 2013-02-05
Answer
You can see on the product datasheet, A13011 anti-MRPL14 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-02-05