Anti-MRPL32 Antibody

Product Name

Anti-MRPL32 Antibody

View all MRPL32 Antibodies

SKU/Catalog Number

A14134

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-MRPL32 Antibody (Catalog # A14134). Tested in WB, IF applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MRPL32 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14134)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human MRPL32.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A14134 is reactive to MRPL32 in Human

Applications

A14134 is guaranteed for IF, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

21.405kDa

Background of MRPL32

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L32 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome Xp.

Suzuki T., J. Biol. Chem. 276:21724-21736(2001).
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Kenmochi N., Genomics 77:65-70(2001).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Immunofluorescence: 1:100~1:500

Validation Images & Assay Conditions

Click image to see more details

Western blot analysis of extracts from Jurkat cells, MCF-7 cells and HepG2 cells, using MRPL32 antibody A14134.

Click image to see more details

Immunofluorescence analysis of A549 cells, using MRPL32 antibody A14134.

Gene/Protein Information For MRPL32 (Source: Uniprot.org, NCBI)

Alternative Names

39S ribosomal protein L32, mitochondrial; HSPC283; L32mtMRP-L32bMRP-59b; mitochondrial ribosomal protein L32 MRPL32 HSPC283, L32mt, MRP-L32, bMRP-59b mitochondrial ribosomal protein L32 39S ribosomal protein L32, mitochondrial|mitochondrial large ribosomal subunit protein bL32m

For more info on MRPL32, check out the MRPL32 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MRPL32: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the MRPL32 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis