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Product Info Summary
| SKU: | A04413-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC-P, WB |
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Product info
Product Name
Anti-MSRA Antibody (N-term)
SKU/Catalog Number
A04413-1
Size
80 µl
Description
Boster Bio Anti-MSRA Antibody (N-term) (Catalog # A04413-1). Tested in IF, Flow Cytometry, IHC-P, WB application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-MSRA Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04413-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This MSRA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 34-63 amino acids from the N-terminal region of human MSRA.
Reactive Species
A04413-1 is reactive to MSRA in Human, Mouse
Calculated molecular weight
26.1 kDa
Background of MSRA
MSRA is ubiquitous and highly conserved. This protein carries out the enzymatic reduction of methionine sulfoxide to methionine. Human and animal studies have shown the highest levels of expression in kidney and nervous tissue. The protein's proposed function is the repair of oxidative damage to proteins to restore biological activity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04413-1 is guaranteed for Flow Cytometry, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:100
WB: 1:1000
IHC-P: 1:10-1:50
FC: 1:10-1:50
Validation Images & Assay Conditions
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Immunofluorescent analysis of A549 cells, using MSRA Antibody (N-term). A04413-1 was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor® 554 (red) conjugated Phalloidin (red).
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Immunofluorescent analysis of A549 cells
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Anti-MSRA Antibody (N-term) at 1:1000 dilution + human kidney lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 26 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Western blot analysis of MSRA antibody (N-term) in mouse kidney tissue lysates (35ug/lane). MSRA (arrow) was detected using the purified Pab.
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Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with MSRA Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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MSRA Antibody (N-term) flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Specific Publications For Anti-MSRA Antibody (N-term) (A04413-1)
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