Product Info Summary
SKU: | A00859 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-MST1/2 (M178) STK4 Antibody
SKU/Catalog Number
A00859
Size
100ul
Form
Liquid
Description
Boster Bio Anti-MST1/2 (M178) STK4 Antibody catalog # A00859. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MST1/2 (M178) STK4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00859)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human BMP-7.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00859 is reactive to STK4 in Human, Mouse, Rat
Applications
A00859 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
55.63kDa
Background of Stk4
Binds double-stranded DNA. Binds preferentially to supercoiled DNA and cruciform DNA structures. Seems to be involved in transcriptional regulation. May function as a transcriptional repressor. Could have a role in the regulation of hematopoietic differentiation through activation of unknown target genes. Controls cellular proliferation by modulating the functions of cell cycle regulatory factors including p53/TP53 and the retinoblastoma protein. May be involved in TP53-mediated transcriptional activation by enhancing TP53 sequence-specific DNA binding and modulating TP53 phosphorylation status. Seems to be involved in energy-level-dependent activation of the ATM/ AMPK/TP53 pathway coupled to regulation of autophagy. May be involved in regulation of TP53-mediated cell death also involving BRCA1. May be involved in the senescence of prostate epithelial cells. Involved in innate immune response by recognizing viral dsDNA in the cytosol and probably in the nucleus. After binding to viral DNA in the cytoplasm recruits TMEM173/STING and mediates the induction of IFN-beta. Has anti-inflammatory activity and inhibits the activation of the AIM2 inflammasome, probably via association with AIM2. Proposed to bind viral DNA in the nucleus, such as of Kaposi's sarcoma-associated herpesvirus, and to induce the formation of nuclear caspase-1-activating inflammasome formation via association with PYCARD. Inhibits replication of herpesviruses such as human cytomegalovirus (HCMV) probably by interfering with promoter recruitment of members of the Sp1 family of transcription factors. Necessary to activate the IRF3 signaling cascade during human herpes simplex virus 1 (HHV-1) infection and promotes the assembly of heterochromatin on herpesviral DNA and inhibition of viral immediate-early gene expression and replication.
Trapani J.A., Immunogenetics 36:369-376(1992).
Trapani J.A., Immunogenetics 40:415-424(1994).
Jiang C., Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunohistochemistry validation of STK4 using Anti-MST1/2 (M178) STK4 Antibody (A00859).
Immunohistochemistry (IHC) analyzes of MST1/2 (M178) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.For more protocol information of IHC
Click image to see more details
Figure 1. Western blotting validation for Anti-MST1/2 (M178) STK4 Antibody A00859
Western blot (WB) analysis of MST1/2 (M178) polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysate
Lane2:Mouse spleen tissue lysate
Lane3:H9C2 whole cell lysate
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For STK4 (Source: Uniprot.org, NCBI)
Gene Name
STK4
Full Name
Serine/threonine-protein kinase 4
Weight
55.63kDa
Superfamily
protein kinase superfamily
Alternative Names
EC 2.7.11; EC 2.7.11.1; kinase responsive to stress 2; KRS2KRS2)); Mammalian STE20-like protein kinase 1; mammalian sterile 20-like 1; MST-1; MST1DKFZp686A2068; serine/threonine kinase 4; serine/threonine-protein kinase 4; Serine/threonine-protein kinase Krs-2; STE20-like kinase MST1 STK4 KRS2, MST1, YSK3 serine/threonine kinase 4 serine/threonine-protein kinase 4|STE20-like kinase MST1|hippo homolog|kinase responsive to stress 2|mammalian STE20-like protein kinase 1|mammalian sterile 20-like 1|serine/threonine-protein kinase Krs-2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on STK4, check out the STK4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for STK4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MST1/2 (M178) STK4 Antibody (A00859)
Hello CJ!
No publications found for A00859
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