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- Table of Contents
MT-CO2 antibody (A03631-1) performs well in ELISA, Flow Cytometry, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 4 Publications
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Flow Cytometry analysis of U87 cells using anti-MT-CO2 antibody (A03631-1).
Overlay histogram showing U87 cells stained with A03631-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MT-CO2 Antibody (A03631-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing U87 cells stained with A03631-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MT-CO2 Antibody (A03631-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-MT-CO2 Antibody Picoband®
MT-CO2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IHC, WB |
Tested Applications
MT-CO2 antibody was positively detected by WB in:
human K562 whole cell, human MCF-7 whole cell, human Hela whole cell, human Jurkat whole cell, human A431 whole cell, human Hacat whole cell, rat liver tissue, rat brain tissue, rat small intestine tissue, rat RH35 whole cell, mouse liver tissue, mouse brain tissue, mouse small intestine tissue, mouse Neuro-2a whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
MT-CO2 antibody was positively detected by IHC in:
human colorectal cancer tissue, human glioma tissue, mouse brain tissue, rat brain tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
MT-CO2 antibody was positively detected by FC in:
U87 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.25 μg/ml
Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human, Mouse, Rat
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
ELISA
0.1-0.5 μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Rat
Tested
Tested
Recommend
Recommend
Mouse
Tested
Tested
Recommend
Recommend
Product Information
Product Name
Anti-MT-CO2 Antibody Picoband®
View all MT-CO2 Antibodies
View all MT-CO2 Antibodies
Form
Lyophilized
Uniprot
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human MT-CO2 recombinant protein (Position: M1-S205).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
21 kDa
Calculated Molecular Weight
25.6 kDa
Background of MT-CO2
Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1.
Cite This Product
Anti-MT-CO2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03631-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Dog
76%
Moderate Homology
Rabbit
74%
Moderate Homology
Cow
74%
Moderate Homology
Mouse
73%
Moderate Homology
Zebrafish
68%
Low homology
Drosophila
59%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for MT-CO2 antibody
Immunohistochemistry (IHC) protocol for MT-CO2 antibody
Flow Cytometry (FC) protocol for MT-CO2 antibody
Publications
Application:
Species:
Effect of environmental tobacco smoke on COX-2 and SHP-2 expression in a periodontitis rat model
Published: 2020-07-08
Journal: ORAL DISEASES
Application: IHC
Impact Factor: 2.61
Species: Rat
PMID: 32640491
Effect of maternal folic acid supplementation on prostatitis risk in the rat offspring
Published: 2018-08-24
Journal: INTERNATIONAL UROLOGY AND NEPHROLOGY
Application: IHC
Impact Factor: 1.69
Species: Rat
PMID: 30141123
Anti-inflammatory effect of tranexamic acid against trauma-hemorrhagic shock-induced acute lung injury in rats
Published: 2018-02-02
Journal: EXPERIMENTAL ANIMALS
Application: IHC
Impact Factor: 1.17
Species: Rat
PMID: 29398669
Interactive actions of prostaglandin and epidermal growth factor to enhance proliferation of granulosa cells from chicken prehierarchical follicles
Published: 2007-02-14
Journal: PROSTAGLANDINS & OTHER LIPID MEDIATORS
Application: ICC
Impact Factor: 1.95
Species: Chicken
PMID: 17499748
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Product Promise
We promise Anti-MT-CO2 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A03631-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 13, 2026