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Product Info Summary
| SKU: | M06465 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human, Mouse |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-MTHFD2 Antibody (C-term)
SKU/Catalog Number
M06465
Size
50 µl
Description
Boster Bio Anti-MTHFD2 Antibody (C-term) (Catalog # M06465). Tested in WB application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-MTHFD2 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06465)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1985CT220.118.31
Isotype
IgG1,κ
Immunogen
This MTHFD2 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 266-299 amino acids from the C-terminal region of human MTHFD2.
Reactive Species
M06465 is reactive to MTHFD2 in Human, Mouse
Calculated molecular weight
37.9 kDa
Background of MTHFD2
Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (By similarity).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06465 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:4000
Validation Images & Assay Conditions
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All lanes : Anti-MTHFD2 Antibody (C-term) at 1:4000 dilution
Lane 1: Hela whole cell lysate
Lane 2: 293 whole cell lysate
Lane 3: MOLT-4 whole cell lysate
Lane 4: Mouse heart lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 38 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Western blot analysis of MTHFD2 using anti-MTHFD2 Antibody (M06465).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTHFD2 antigen affinity purified polyclonal antibody (M06465) at 1:2000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:2500 dilution at RT for 1 hour. The signal is developed using an Fluorescence. A specific band was detected for MTHFD2 at approximately 40 kDa. The expected band size for MTHFD2 is at 40 kDa.
Specific Publications For Anti-MTHFD2 Antibody (C-term) (M06465)
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Customer Reviews
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1 Reviews For Anti-MTHFD2 Antibody (C-term)
MTHFD2 Antibody for Western Blot
Excellent
Source: Biocompare.com
| SKU | M06465 |
|---|---|
| Application | Western Blot |
| Sample | Hello cell and mouse tissues |
| Primary Incubation | 1:2000 dilution at 4oC overnight |
| Blocking Agent | 5% non-fat milk |
| Secondary Incubation | 1:2500 dilution at RT for 1 hour |
| Detection | Fluorescence |
| Results Summary | This antibody works in WB with tested samples. The signal is strong enough to get a decent image. It works for human and mouse samples. |
"This antibody of MTHFD2 is good for WB with mouse tissue and human cell. Signal is strong and the background is ok."
Hua Jiang
Verified customer
Submitted 2024-07-15
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