Anti-MURF1/TRIM63 Antibody Picoband™

Product Name

Anti-MURF1/TRIM63 Antibody Picoband™

View all MuRF1/TRIM63 Antibodies

SKU/Catalog Number

A02016-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-MURF1/TRIM63 Antibody Picoband™ catalog # A02016-2. Tested in ELISA, WB applications. This antibody reacts with Human, Monkey.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MURF1/TRIM63 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02016-2)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human MURF1/TRIM63 recombinant protein (Position: D2-E329).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A02016-2 is reactive to TRIM63 in Human, Monkey

Applications

A02016-2 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

40 kDa

Calculated molecular weight

40.248kDa

Background of MuRF1/TRIM63

E3 ubiquitin-protein ligase TRIM63 is an enzyme that in humans is encoded by the TRIM63 gene. This gene encodes a member of the RING zinc finger protein family found in striated muscle and iris. The product of this gene is an E3 ubiquitin ligase that localizes to the Z-line and M-line lattices of myofibrils. This protein plays an important role in the atrophy of skeletal and cardiac muscle and is required for the degradation of myosin heavy chain proteins, myosin light chain, myosin binding protein, and for muscle-type creatine kinase.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Monkey
Direct ELISA, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of MURF1/TRIM63 using anti-MURF1/TRIM63 antibody (A02016-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: monkey heart tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MURF1/TRIM63 antigen affinity purified polyclonal antibody (Catalog # A02016-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MURF1/TRIM63 at approximately 40KD. The expected band size for MURF1/TRIM63 is at 40KD.

Gene/Protein Information For TRIM63 (Source: Uniprot.org, NCBI)

Alternative Names

EC 6.3.2.-; IRF; IRFMURF2; Iris RING finger protein; MuRF1; MuRF-1; muscle specific ring finger protein 2; Muscle-specific RING finger protein 1; RING finger protein 28FLJ32380; RNF28; RNF28E3 ubiquitin-protein ligase TRIM63; SMRZ; SMRZMURF-1; Striated muscle RING zinc finger protein; TRIM63; tripartite motif containing 63; tripartite motif-containing 63; Tripartite motif-containing protein 63 TRIM63 IRF, MURF1, MURF2, RNF28, SMRZ tripartite motif containing 63 E3 ubiquitin-protein ligase TRIM63|RING-type E3 ubiquitin transferase TRIM63|iris ring finger protein|muscle specific ring finger protein 2|muscle-specific RING finger protein 1|ring finger protein 28|striated muscle RING zinc finger protein|tripartite motif containing 63, E3 ubiquitin protein ligase|tripartite motif-containing protein 63

For more info on TRIM63, check out the TRIM63 Infographic

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In this infographic, you will see the following information for TRIM63: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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