Product Info Summary
SKU: | A00810-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-MUS81 Antibody Picoband™
SKU/Catalog Number
A00810-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MUS81 Antibody Picoband™ catalog # A00810-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-MUS81 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00810-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human MUS81 recombinant protein (Position: R6-C545).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00810-1 is reactive to Mus81 in Human, Rat
Applications
A00810-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
70 kDa
Calculated molecular weight
61.531kDa
Background of Mus81
Crossover junction endonuclease MUS81 is an enzyme that in humans is encoded by the MUS81 gene. This gene encodes a structure-specific endonuclease which belongs to the XPF/MUS81 endonuclease family and plays a critical role in the resolution of recombination intermediates during DNA repair after inter-strand cross-links, replication fork collapse, and DNA double-strand breaks. The encoded protein associates with one of two closely related essential meiotic endonuclease proteins (EME1 or EME2) to form a complex that processes DNA secondary structures. It contains an N-terminal DEAH helicase domain, an excision repair cross complementation group 4 (ERCC4) endonuclease domain, and two tandem C-terminal helix-hairpin-helix domains. Mice with a homozygous knockout of the orthologous gene have significant meiotic defects including the failure to repair a subset of DNA double strand breaks.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Rat
Flow Cytometry, 1-3 μg/1x106 cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of MUS81 using anti-MUS81 antibody (A00810-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MUS81 antigen affinity purified polyclonal antibody (Catalog # A00810-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MUS81 at approximately 70 kDa. The expected band size for MUS81 is at 61 kDa.
Click image to see more details
Figure 2. Flow Cytometry analysis of 293T cells using anti-MUS81 antibody (A00810-1).
Overlay histogram showing 293T cells stained with A00810-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MUS81 Antibody (A00810-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For Mus81 (Source: Uniprot.org, NCBI)
Gene Name
Mus81
Full Name
Crossover junction endonuclease MUS81
Weight
61.531kDa
Superfamily
XPF family
Alternative Names
crossover junction endonuclease MUS81; EC 3.1.22; EC 3.1.22.-; FLJ21012; FLJ44872; MUS81 endonuclease homolog (S. cerevisiae); MUS81 endonuclease homolog (yeast); SLX3 structure-specific endonuclease subunit homolog; SLX3 Mus81|1200008A18Rik, AI182501, AW045863|MUS81 structure-specific endonuclease subunit|crossover junction endonuclease MUS81|MUS81 endonuclease homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Mus81, check out the Mus81 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Mus81: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MUS81 Antibody Picoband™ (A00810-1)
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